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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Banana resistant to Fusarium Wilt Tropical Race 4
EN
QCAV-4
Yes
QUT-QCAV4-6
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Organization:Queensland University of Technology (QUT)Academic or research instituteGPO Box 2434Brisbane, Queensland
4001, AustraliaPhone: +(61) (07) 3138 2000,Fax:Email: hdr@qut.edu.au,Website: https://www.qut.edu.au/,
Musa acuminata subgroup Cavendish cultivar Grand Nain was modified to confer resistance to Fusarium oxysporum (f. sp.) cubense Tropical Race 4, the causal agent of Fusarium wilt. To achieve disease resistance, the LM banana expresses the MamRGA2 gene from Musa acuminata ssp. malaccensis, which functions as an intracellular immune receptor that recognizes pathogen effector molecules and activates a cascade of defense responses, including pathogenesis-related protein expression and programmed cell death. The LM banana also contains the neomycin phosphotransferase type II gene derived from Escherichia coli as a selectable marker, conferring resistance to antibiotics kanamycin and/or neomycin. Regulatory sequences used to control expression of the introduced genes were derived from Agrobacterium tumefaciens and the Cauliflower mosaic virus.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-280159-1 Organism Musa acuminata (Banana, Plantain, Cavendish banana, Dessert banana, Chiquita banana, Grand Naine, Grand Nain, Musa acuminata Colla, Umalag, Pisang Masak Hijau, Gros Michel)Crops
Grand Nain
EN
pSAN3
EN
- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-280167-1 Resistance analogue gene 2 | Musa acuminata subsp. malaccensis (Wild Malaysian banana, True apple banana, Nasution, Musa malaccensis Ridl, Pisang hutan, Pisang karok)Protein coding sequence | Resistance to diseases and pests (Fungi)
The modified banana contains two genetic cassettes driving the expression of MamRGA2 (Musa acuminata ssp. malaccensis resistance analogue 2) and nptII (neomycin phosphotransferase II) genes.
MamRGA2 gene is regulated by the nopaline synthase gene promoter from Agrobacterium tumefaciens and nopaline synthase gene terminator.
The nptII gene expression is directed by the 35S promoter from Cauliflower mosaic virus (CaMV) and terminated by the 35S terminator by CaMV.
MamRGA2 gene is regulated by the nopaline synthase gene promoter from Agrobacterium tumefaciens and nopaline synthase gene terminator.
The nptII gene expression is directed by the 35S promoter from Cauliflower mosaic virus (CaMV) and terminated by the 35S terminator by CaMV.
Notes:
- A 26,849 bp DNA fragment was inserted at a single site on chromosome 6 in the antisense orientation in an intergenic region. The insertion resulted in a 116 bp deletion at the site but did not disrupt any known open reading frames (ORFs);
- The insert includes:
- Three identical full-length T-DNA copies (6,702 bp each);
- Two truncated fragments of the MamRGA2 gene recombined in opposite directions between T-DNA2 and T-DNA3;
- Small genetic changes such as indels or point mutations between the flanking regions and insert as well as between the intact copies of the introduced gene cassette.
- PCR and whole-genome sequencing confirmed no integration of plasmid backbone sequences;
- Seven novel ORFs (>30 amino acids) were identified within the insert, but RNA-Seq analysis showed no transcriptional activity in leaf, root, and fruit tissues;
- RNA-Seq from leaf, root, and fruit tissue showed no transcriptional activity of these ORFs;
- Southern blot analysis over four successive generations;
- MamRGA2 expression was confirmed via RT-qPCR, and higher expression levels correlated with increased resistance to TR4.
EN
- Food
EN
EN
- QCAV-4, the first genetically modified Cavendish (cv. Grand Nain) banana resistant to Fusarium wilt tropical race 4 approved for commercial production and consumption [ English ]
- QCAV-4, the first genetically modified Cavendish (cv. Grand Nain) banana resistant to Fusarium wilt tropical race 4 approved for commercial production and consumption [PDF] [ English ]
- Australian Office of the Gene Technology Regulator - DIR 199 - Commercial release of banana genetically modified for resistance to Fusarium wilt tropical race 4 (TR4) [URL] [ English ]
- Australian Office of the Gene Technology Regulator - DIR 199 - Full risk assessment and risk management plan [PDF] ( A1274 ) [ English ]
- FSANZ - Application for QCAV-4 LM banana inclusion in Standard 1.5.2 [PDF] ( amendment, Australia New Zealand Food Standards Code, Food Produced Using Gene Technology ) [ English ]
- FSANZ - Safety assessment for food derived from disease-resistant banana line QCAV-4 [PDF] ( Application A1274 ) [ English ]
- Euginius - QCAV-4 banana detailed information (QUT-QCAV4-6) [URL] [ English ]
- OECD BioTrack Product Database: QUT-QCAV4-6 [URL] [ English ]
| Record type | Field | Record(s) | |
|---|---|---|---|
| Country's Decision or any other Communication | LMO identification | 1 | |
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 1 | |
