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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Roundup Ready™ Enlist™ maize
EN
DAS40278 x NK603
Yes
DAS-4Ø278-9 x MON-ØØ6Ø3-6
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Organization:Corteva Agriscience ()Private sector (business and industry)9330 Zionsville RoadIndianapolis, Indiana
46268, United States of AmericaPhone: +1 833-267-8382,Fax:Email: media@corteva.com,Website: https://www.corteva.com/,
The maize (Zea mays) was produced through cross breeding of modified parental varieties NK603 and DAS40278. For tolerance to glyphosate, the maize expresses Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate, which has a low binding affinity for the herbicidal compound and allows for the continued synthesis of aromatic amino acids through the shikimate pathway. For tolerance to aryloxyphenoxypropionate and 2,4-dichlorophenoxyacetic acid herbicides (acetyl‐CoA carboxylase inhibitors), the modified maize expresses Sphingobium herbicidovorans aryloxyalkanoate dioxygenase (AAD-1), which cleaves 2,4-dichlorophenoxyacetic acid into non-herbicidal dichlorophenol and glyoxylate, as well as inactivates aryloxyphenoxypropionate herbicides (acetyl‐CoA carboxylase inhibitors).
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)Crops
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BCH-LMO-SCBD-14776-18 Living Modified Organism MON-ØØ6Ø3-6 - Roundup Ready™ maizeMonsanto | Resistance to herbicides (Glyphosate)
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BCH-LMO-SCBD-104814-2 Living Modified Organism DAS-4Ø278-9 - Enlist™ MaizeDow AgroSciences GmbH | Resistance to herbicides, Tolerance to 2,4-Dichlorophenoxyacetic acid, Tolerance to aryloxyphenoxypropionate
EN
PV-ZMGT32 and pDAS1740
EN
- Biolistic / Particle gun
- Cross breeding
- Other (silicon carbide whisker fibres)
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0.800 kb
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0.600 kb
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0.200 kb
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1.400 kb
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0.800 kb
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1.400 kb
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1.166 kb
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1.991 kb
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0.891 kb
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0.365 kb
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1.166 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-104795-4 RB7 matrix attachment region | Nicotiana tabacum (Tobacco, TOBAC )Enhancer
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BCH-GENE-SCBD-100359-7 Hsp70 intron | Zea mays (Maize, Corn, MAIZE)Intron
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
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BCH-GENE-SCBD-100364-5 Rice actin 1 gene promoter | Oryza sativa (Rice, ORYSA)Promoter
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BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)Protein coding sequence | Resistance to herbicides (Glyphosate)
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BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Transit signal
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-104812-3 Aryloxyalkanoate dioxygenase gene | Sphingobium herbicidovorans (SPHHE)Protein coding sequence | Resistance to herbicides,Tolerance to 2,4-Dichlorophenoxyacetic acid,Tolerance to aryloxyphenoxypropionate
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BCH-GENE-SCBD-104813-4 Per5 3' Untranslated Region | Zea mays (Maize, Corn, MAIZE)Terminator
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BCH-GENE-SCBD-100355-6 Rice actin 1, intron | Oryza sativa (Rice, ORYSA)Intron
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
DNA insert from NK603 (vector PV-ZMGT32)
The parental maize contributes two adjacent genetic cassettes for Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (cp4-epsps).
In the first (5' end) expression cassette, the cp4-epsps gene is under the transcriptional regulation of an Oryza sativa actin promoter and an A. tumefaciens nopaline synthase gene (nos) terminator. An O. sativa actin intron is also present in the transcript for enhanced expression of the coding sequence. The second cassette consists of another cp4-epsps gene regulated by a Cauliflower mosaic virus enhanced 35S promoter (containing a duplicated enhancer region) and a nos terminator. Similarly, an intron from the Zea mays heat shock protein 70 (hsp70) was included for enhancing expression of the coding sequence. Both promoters of the gene cassettes are expected to promote high levels of transcription.
Note:
The parental maize contributes two adjacent genetic cassettes for Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (cp4-epsps).
In the first (5' end) expression cassette, the cp4-epsps gene is under the transcriptional regulation of an Oryza sativa actin promoter and an A. tumefaciens nopaline synthase gene (nos) terminator. An O. sativa actin intron is also present in the transcript for enhanced expression of the coding sequence. The second cassette consists of another cp4-epsps gene regulated by a Cauliflower mosaic virus enhanced 35S promoter (containing a duplicated enhancer region) and a nos terminator. Similarly, an intron from the Zea mays heat shock protein 70 (hsp70) was included for enhancing expression of the coding sequence. Both promoters of the gene cassettes are expected to promote high levels of transcription.
Note:
- The parental NK603 line contained a single, intact insertion containing both cp4-epsps gene cassettes.
- Due to a restriction digest prior to particle bombardment, the vector backbone, containing Escherichia coli neomycin phosphotransferase II and origin of replication, were not incorporated into the parental genome.
DNA insert from DAS40278 (DAS-4Ø278-9) vector pDAS1740
The parental LMO was generated using the Whiskers-mediated transformation method and contains a Sphingobium herbicidovorans aryloxyalkanoate dioxygenase-1 (aad-1) gene cassette.
The aad-1 coding sequence is under the control of a Zea mays ubiquitin gene promoter and Z. mays root preferential cationic peroxidase terminator. Due to the constitutive nature of the ubiquitin promoter, elevated levels of transcription of this gene cassette are expected to occur.
Note:
- The aad-1 coding sequence was optimized for expression in the plant.
- Southern blot analysis indicated that a single complete copy of the transformation cassette was stably integrated into the host genome at a single locus.
- No integration of the vector backbone occurred.
For more information, kindly refer to the parental LMO records.
EN
- Feed
- Food
EN
EN
- Argenbio Maíz DP-2Ø2216-6 x MON-ØØ6Ø3-6 x DAS-4Ø278-9 y combinaciones intermedias [ Spanish ]
- Import-and-processing-of-gm-maize-DP202216xNK603xDAS-40278-9.pdf ( NK603, DAS-40278-9, maize, sub-combinations ) [ English ]
- Euginius DAS40278 x NK603 [ English ]
- OECD BioTrack Product Database MON-ØØ6Ø3-6 [ English ]
- OECD BioTrack Product Database DAS-4Ø278-9 [ English ]
| Record type | Field | Record(s) | |
|---|---|---|---|
| Country's Decision or any other Communication | LMO identification | 1 | |
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 1 | |
