The soybean (Glycine max) was modified to confer protection against lepidopteran pests and resistance to acetolactate synthase (ALS)-inhibiting herbicides. To achieve protection against susceptible lepidoptera, the LM soybean expresses the insecticidal proteins encoded by the Cry genes modified from Bacillus thuringiensis, cry1B.34.1 and cry1B.61.1, and the Adiantum trapeziforme insecticidal protein gene, ipd083Cb. The Cry proteins function as insecticidal toxins that, when ingested, are proteolytically activated in the midgut of the insects and bind to specific receptors on epithelial cells, forming pores that lead to insect death. The mode of action of the IPD083Cb protein is similar to that of Cry proteins, the IPD083Cb protein binds to specific sites in the midgut of susceptible insects, causing the insects to die. To achieve herbicide resistance, the LM soybean expresses the gm-hra_1 gene which is a variant of the acetolactate synthase enzyme derived from Glycine max (also known as acetohydroxy acid synthase). This protein contains two mutations in its amino acid sequence that reduce binding affinity to ALS-inhibiting herbicides and thus are responsible for GM-HRA resistance to ALS-inhibiting herbicides (e.g., sulfonylureas and triazolopyrimidines) and was used as a selection marker during the development of the transformation event. Four expression cassettes were incorporated into the modified soybean: three expressing the insecticidal proteins to confer both insect resistance, and one cassette expressing the GM-HRA protein to confer herbicide resistance and used as a marker selection.