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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Insect-resistant and herbicide-tolerant soybean
EN
DBN-08205-8
Yes
DBN-Ø82Ø5-8
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Organization:Beijing Dabeinong Biotechnology Co., Ltd. (DBNBC)Private sector (business and industry)No. 2, Old Summer Palace West RoadBeijing, Haidan
100193, ChinaPhone: +86 010-82408600,Fax: +86 010-82408644,Email: hr-biotech@dbn.com.cn, cwbgs@dbn.com.cn,Website: http://www.dbnbc.cn/en,
The soybean (Glycine max) was modified for resistance to lepidopteran insects and tolerance to glufosinate herbicides. For resistance to lepidopteran pests, the soybean expresses the Bacillus thuringiensis Cry1Ac and Cry2Ab insecticidal crystal proteins, which form non-specific ion-conducting pores in the apical membrane of midgut epithelial cells, leading to disruption of the midgut epithelium and eventual death of susceptible insects. For tolerance to glufosinate, the soybean expresses phosphinothricin N-acetyltransferase (PAT), which inactivates the herbicide through acetylation.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-10453-8 Organism Glycine max (Soybean, Soya bean, Soya / soja /соя, SOYBN)Crops
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pDBN4031
EN
- Agrobacterium-mediated DNA transfer
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1.408 kb
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0.228 kb
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1.905 kb
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0.643 kb
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1.723 kb
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0.264 kb
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3.537 kb
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0.253 kb
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0.530 kb
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0.552 kb
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0.195 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-14986-6 Cry1Ac | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-GENE-SCBD-14988-7 Cry2Ab2 | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-GENE-SCBD-15002-5 Phosphinothricin N-acetyltransferase | Streptomyces viridochromogenes (STRVR)Protein coding sequence | Resistance to herbicides (Glufosinate)
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BCH-GENE-SCBD-104517-2 Actin 2 promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Promoter
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BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Transit signal
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BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)Terminator
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BCH-GENE-SCBD-103851-5 rbcS Promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Promoter
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BCH-GENE-SCBD-101902-5 Ribulose-1,5-bisphosphate carboxylase small subunit transit peptide | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Transit signal
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
Information on the inserted DNA sequences
The transforming plasmid pDBN4031 contained a transfer DNA (T-DNA) region comprising three gene cassettes expressing the insecticidal proteins Cry2Ab2 and Cry1Ac derived from Bacillus thuringiensis and the herbicide tolerance protein phosphinothricin N-acetyltransferase (PAT). Transformation of soybean was achieved through Agrobacterium tumefaciens-mediated transformation.
(1) Transcription of the cry2Ab2 coding sequence is directed by the Actin 2 promoter from Arabidopsis thaliana. The coding sequence is preceded by the CTP2 chloroplast transit peptide sequence from Arabidopsis thaliana, which directs the expressed protein to the chloroplast. Transcription is terminated by the rbcS E9 terminator from Pisum sativum. Expression of this cassette results in production of the Cry2Ab2 insecticidal crystal protein, which confers resistance to susceptible lepidopteran insect pests.
(2) Transcription of the cry1Ac coding sequence is directed by the rbcS promoter from Arabidopsis thaliana. The coding sequence is preceded by the rbcS chloroplast transit peptide sequence from Arabidopsis thaliana, which directs the expressed protein to the chloroplast. Transcription is terminated by the nopaline synthase terminator from Agrobacterium tumefaciens. Expression of this cassette results in production of the Cry1Ac insecticidal crystal protein, which confers resistance to susceptible lepidopteran insect pests.
(3) Transcription of the pat coding sequence is directed by the Cauliflower mosaic virus 35S promoter. Transcription is terminated by the Cauliflower mosaic virus 35S terminator. Expression of this cassette results in production of the phosphinothricin N-acetyltransferase (PAT) protein, which confers tolerance to glufosinate herbicides by inactivating the herbicide through acetylation.
Notes:
Molecular characterization demonstrated that the genome of DBN8205 soybean contains a single, intact copy of the cry1Ac, cry2Ab2 and pat expression cassettes.
Molecular characterization demonstrated that the genome of DBN8205 soybean contains a single, intact copy of the cry1Ac, cry2Ab2 and pat expression cassettes.
Southern blot analysis confirmed the absence of integrated plasmid backbone sequences.
The inserted DNA was shown to be stably integrated into the soybean genome and stably inherited across and within breeding generations.
The inserted DNA was shown to be stably integrated into the soybean genome and stably inherited across and within breeding generations.
EN
- Feed
- Food
- EUginius DBN8205 [ English ]
- Development and Validation of Event-Specific PCR Method for the Quantification of Genetically Modified Soybean DBN8205.pdf ( DBN8205, real-time quantitative PCR, duplex digital PCR, quantification ) [ Chinese ]
- Development and Validation of Event-Specific PCR Method for the Quantification of Genetically Modified Soybean DBN8205 [ Chinese ]
EN
EN
- ISAAA database - DBN-08205-8 [ English ]
- Argentina - Resolución 169/2025 [ Spanish ]
- WO2023155193A1 Patent.pdf ( Patent, DBN8205, molecular characterization, soybean ) [ Chinese ]
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