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Living Modified Organism
(LMO)
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Potato with modified starch composition
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AM02-1003, AM02-1005, AM02-1012, AM02-1017 and AM99-1089.
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Person:BASF Plant Science GmbHCarl-Bosch-Str. 38Ludwigshafen,
67056 , GermanyPhone: +49 621 60-0,Fax: +49 621 60-42525,Email: global.info@basf.com,
A gene modifying the starch composition in potato tubers raising the level of amylopectin has been introduced into potato. The neomycin phophotransferase II gene or an acetohydroxyacid synthase gene have been used as selectable marker genes.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12106-6 Organism Solanum tuberosum (Potato, SOLTU)Crops
Potato variety Seresta (lines AM02-1003, AM01-1005, AM02-1012) and Kuras (line AM02-1017)
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Derived from pZPZ200 for construct pAP2 and pGPTV-KAN for construct pHAxwO
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-48072-3 Granule-bound starch synthase gene | Solanum tuberosum (Potato, SOLTU)Protein coding sequence | altered carbohydrate composition: increased amylopectin content
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BCH-GENE-SCBD-14997-6 Granule bound starch synthase gene promoter | Solanum tuberosum (Potato, SOLTU)Promoter
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-48073-8 Acetohydroxy acid synthase gene | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Protein coding sequence | Resistance to herbicides (Imidazolinone, Sulfonylurea)
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-103067-9 Transcript 7 gene 3' untranslated region | Agrobacterium tumefaciens (Agrobacterium)Terminator
Inserted DNA
a) A gene containing the coding region of potato gbss in antisense orientation relative to the promoter, flanked by the the gbss promoter from Solanum tuberosum and the polyadenylation sequence from Agrobacterium tumefaciens nopaline synthase gene has been inserted into potato variety Seresta (lines AM02-1003, AM01-1005, AM02-1012) and Kuras (line AM02-1017) thus reducing the amount of amylose in the starch fraction. An ahas gene (acetohydroxyacid synthase) from Arabidopsis thaliana flanked by the Agrobacterium tumefaciens nos gene promoter and the octopine synthase polyadenylation sequence serves as selectable marker gene conferring tolerance to Imazamox.
b) AM99-1089 serves as a reference line. The inserted gene consists of the potato gbss (granule bound starch synthase) promoter, the coding region of potato gbss in antisense orientation and the polyadenylation sequence from Agrobacterium tumefaciens nopaline synthase gene thus reducing the amount of amylose in the starch fraction. In addition the neomycin phosphotransferase gene (nptII) connected to the Agrobacterium tumefaciens nopaline synthase promoter and g7 polyadenylation sequence from Agrobacterium tumefaciens has been inserted as selectable marker gene conferring resistance to kanamycin.
EN
a) A gene containing the coding region of potato gbss in antisense orientation relative to the promoter, flanked by the the gbss promoter from Solanum tuberosum and the polyadenylation sequence from Agrobacterium tumefaciens nopaline synthase gene has been inserted into potato variety Seresta (lines AM02-1003, AM01-1005, AM02-1012) and Kuras (line AM02-1017) thus reducing the amount of amylose in the starch fraction. An ahas gene (acetohydroxyacid synthase) from Arabidopsis thaliana flanked by the Agrobacterium tumefaciens nos gene promoter and the octopine synthase polyadenylation sequence serves as selectable marker gene conferring tolerance to Imazamox.
b) AM99-1089 serves as a reference line. The inserted gene consists of the potato gbss (granule bound starch synthase) promoter, the coding region of potato gbss in antisense orientation and the polyadenylation sequence from Agrobacterium tumefaciens nopaline synthase gene thus reducing the amount of amylose in the starch fraction. In addition the neomycin phosphotransferase gene (nptII) connected to the Agrobacterium tumefaciens nopaline synthase promoter and g7 polyadenylation sequence from Agrobacterium tumefaciens has been inserted as selectable marker gene conferring resistance to kanamycin.
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- Food
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