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Living Modified Organism
(LMO)
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Pea resistant to viral diseases
EN
pWell05, pWell08, pWell07
No
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Person:Agritec Plant ResearchZemedelska 2520/16Sumperk,
787 01, CzechiaPhone: +420 583 382-111,Fax: +420 583 382-999,Email: info@agritec.cz,Website: http://agritec.cz/new/index.php?lang=en,Related OrganizationAgritec Plant Research ()Private sector (business and industry)Zemedelska 2520/16Sumperk,
787 01, CzechiaPhone: +420 583 382-111,Fax: +420 583 382-999,Email: info@agritec.cz,Website: http://agritec.cz/new/index.php?lang=en,
Pea plans were modifies for resistance to the viral pathogens Pea Enation Mosaic Virus (PEMV) or Pea Seed-borne Mosaic Virus (PSbMV).
The transformed genotypes contain selectable marker genes bar, conferring resistance to phosphinothricin – active component of Basta herbicide, and GUS for the facilitation of selection of genetically modified tissues and plants.
EN
The transformed genotypes contain selectable marker genes bar, conferring resistance to phosphinothricin – active component of Basta herbicide, and GUS for the facilitation of selection of genetically modified tissues and plants.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-100289-5 Organism Pisum sativum (Garden pea, PEA)Crops
EN
pWell05, pWell08 or pWell07
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-46004-7 Beta-glucuronidase coding sequence | Escherichia coli (ECOLX)Protein coding sequence | Selectable marker genes and reporter genes
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-101930-3 PEMV coat protein | Pea enation mosaic virus 1 (PEMV-1)Protein coding sequence | Resistance to diseases and pests (Viruses, Mosaic virus)
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BCH-GENE-SCBD-101940-3 PSbMV coat protein | Pea seed-borne mosaic virus (PSbMV)Protein coding sequence | Resistance to diseases and pests (Viruses)
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BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)Protein coding sequence | Resistance to herbicides (Glufosinate)
The constructs of pWell05 and 08 lines contain a gene for cpPEMV coat protein.The pWell05 construct contains the whole sequence of the coat protein, whilst the pWell08 construct contains only a fragment of the sequence.
The pWell 07 construct contains a congenerous fragment of cpPSbMV gene for coat protein. The above-mentioned sequences should confer resistance to relevant viral pathogens (pea enation mosaic virus and pea seed-borne mosaic virus).
EN
The pWell 07 construct contains a congenerous fragment of cpPSbMV gene for coat protein. The above-mentioned sequences should confer resistance to relevant viral pathogens (pea enation mosaic virus and pea seed-borne mosaic virus).
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- Research
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