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Living Modified Organism
(LMO)
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Rubber tree modified to express a reporter gene
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HT1
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Person:Dr. Sunderasan ElumalaiResearch Officer, Genetic Transformation and Tissue Culture Programme, Production Advancement DivisionGenetic Transformation and Tissue Culture Programme, Production Advancement Division, Malaysian Rubber Board,Sungai Buloh, Selangor
47000, MalaysiaPhone: +603-61459599,Fax: +603-61565251,Email: sunderasan@lgm.gov.my,Website:Related OrganizationMalaysian Rubber Board (LGM)Genetic Transformation and Tissue Culture Programme, Production Advancement Division, Malaysian Rubber Board,Sungai Buloh, Selangor
47000, MalaysiaPhone: +603-61459599,Fax: +603-61565251,Email: sunderasan@lgm.gov.my,Website:
Hevea brasiliensis was modified to express the glucuronidase (GUS) reporter gene encoding glucuronidase enzyme that hydrolyzes x-gluc - a colourless substrate, into soluble blue coloured product. This serves as an indicator of successful expression of transgene product in the target tissue.
GUS enzyme activity has been detected in latex and leaf tissue of transformed Hevea plants.
Hevea transformed with GUS also harbours the nptII gene that confers resistance to kanamycin, which was incorporated in the growth media to select transformed callus after co-cultivation with Agrobacterium.
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GUS enzyme activity has been detected in latex and leaf tissue of transformed Hevea plants.
Hevea transformed with GUS also harbours the nptII gene that confers resistance to kanamycin, which was incorporated in the growth media to select transformed callus after co-cultivation with Agrobacterium.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-108280-2 Organism Hevea brasiliensis (Rubber tree, Para rubber tree, SiringaTree, Jebe, HEVBR)Trees
Clone GL1
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p35S-GUS-INT
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-108284-1 Beta-Glucuronidase coding sequence | Streptococcus pneumoniae (Pneumococcus, STREE)Protein coding sequence | Selectable marker genes and reporter genes
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
Agrobacterium tumefaciens containing p35S-GUS-INT plant expression vector was a gift from Prof. L. Willmitzer, Institut fur Genbiologische Forschung, Berlin.
The inserted genetic material is the region between the left border and right boarder (T-DNA) from the Ti plasmid of Agrobacterium tumefaciens harbouring the above described gene construct.
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The inserted genetic material is the region between the left border and right boarder (T-DNA) from the Ti plasmid of Agrobacterium tumefaciens harbouring the above described gene construct.
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- Research
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| Record type | Field | Record(s) | |
|---|---|---|---|
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 1 | |
| Country's Decision or any other Communication | LMO identification | 1 | |
