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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Drought tolerant sugarcane
EN
NXI-4T
No
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Surabaya,Organization:PT Perkebunan Nusantara XI (Persero)
60175, IndonesiaPhone: +62 031 3524596,Fax:Email: sekretariat@ptpn11.co.id,Website: https://ptpn11.co.id/,
The sugarcane (Saccharum officinarum) was modified for abiotic (drought, salt) stress tolerance through the expression of Rhizobium meliloti choline dehydrogenase, which leads to increased glycine betaine biosynthesis. Glycine betaine maintains a cell's water potential by osmotic adjustment. The expression of choline dehydrogenase may also increase sugar content and promote early maturing.
The sugarcane also contains Escherichia coli neomycin phosphotransferase II and hygromycin B phosphotransferase for kanamycin and hygromycin resistance, respectively.
The sugarcane also contains Escherichia coli neomycin phosphotransferase II and hygromycin B phosphotransferase for kanamycin and hygromycin resistance, respectively.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-115592-1 Organism Saccharum officinarum L. (Sugarcane, Sugar cane)Crops
Sugarcane cultivar BL579
EN
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Drought tolerant sugarcane| Changes in physiology and/or production (Ripening, Yield), Resistance to antibiotics (Hygromycin, Kanamycin), Selectable marker genes and reporter genes, Tolerance to abiotic stress (Drought, Salinity)
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Drought-tolerant sugarcane| Changes in physiology and/or production (Ripening, Yield), Resistance to antibiotics (Hygromycin, Kanamycin, Neomycin), Selectable marker genes and reporter genes, Tolerance to abiotic stress (Drought, Salinity)
pMLH2113
EN
- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-116036-1 Glutamate dehydrogenase mitochondrial transit peptide | Solanum lycopersicum (Tomato, SOLLC)Transit signal
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BCH-GENE-SCBD-116039-1 Choline dehydrogenase | Sinorhizobium meliloti (RHIML)Protein coding sequence | Changes in physiology and/or production (Ripening, Yield),Tolerance to abiotic stress (Drought, Salinity)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-14991-8 Hygromycin B phosphotransferase gene | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Hygromycin),Selectable marker genes and reporter genes
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-104820-3 Omega 5' untranslated leader | Tobacco mosaic virus (TMV)Leader
The modified sugarcane contains three gene cassettes: Rhizobium meliloti choline dehydrogenase (betA); Escherichia coli neomycin phosphotransferase (nptII) and E. coli hygromycin B phosphotransferae (hph).
The betA sequence is under control of a Cauiflower mosaic virus (CaMV) 35S promoter and Agrobacterium tumefaciens nopaline synthase (nos) terminator. At the 5' end of the betA coding sequence is a Tobacco mosaic virus omega 5' leader to enhance translation and a Solanum lycopersicum glutamate dehydrogenase mitochondrial transit peptide, which directs the translated protein to the mitochondria.
The hph coding sequence is under transcriptional control of a CaMV 35S promoter and terminator.
The nptII coding sequence is under transcriptional control of a nos promoter and terminator.
Important notes:
- The CaMV promoter associated with the betA coding sequence may have two tandem repeats of -419 to -90.
The betA sequence is under control of a Cauiflower mosaic virus (CaMV) 35S promoter and Agrobacterium tumefaciens nopaline synthase (nos) terminator. At the 5' end of the betA coding sequence is a Tobacco mosaic virus omega 5' leader to enhance translation and a Solanum lycopersicum glutamate dehydrogenase mitochondrial transit peptide, which directs the translated protein to the mitochondria.
The hph coding sequence is under transcriptional control of a CaMV 35S promoter and terminator.
The nptII coding sequence is under transcriptional control of a nos promoter and terminator.
Important notes:
- The CaMV promoter associated with the betA coding sequence may have two tandem repeats of -419 to -90.
EN
- Biofuel
- Food
EN
The events NXI-1T and NXT-6T were developed while producing NXT-4T.
- EUginius - NXI-4T sugarcane [ English ]
- ISAAA - NXI-4T sugarcane [ English ]
- Biotechnology_of_Drought-Tolerant_Sugarcane.pdf [ English ]
- Beating the heat (Nature Biotechnology).pdf [ English ]
- Application No AU 200059458 B2 - Early-maturing sugarcane with high sugar content.pdf [ English ]
- Efficient Promoter Cassettes for Enhanced Expression of Foreign Genes in Monocot and Dicot plants.pdf [ English ]
- Sugarcane Water Stress Tolerance Mechanisms and Its Implications on Developing Biotechnology Solutions.pdf [ English ]
- Powerpoint July 2019 - Jember University - Biosafety Certification.pdf [ English ]
| Record type | Field | Record(s) | |
|---|---|---|---|
| Living Modified Organism | Related LMO(s) | 2 | |
