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Living Modified Organism
(LMO)
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Spring barley with altered oxidative-stress response and ozone sensitivity (hv_sheer)
EN
hv_sheer
No
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Klopina,Organization:Usovsko ()Private sector (business and industry)
78973, CzechiaPhone: +420 583 484 111,Fax:Email: klopina@usovsko.cz,Website: http://usovsko.cz/?p=o-nas,
The spring barley (Hordeum vulgare) was modified for an altered oxidative-stress response and sensitivity to ozone. SHEER encodes the Stress-Induced HEmE Receptor, a heme-binding protein implicated in stress signalling and maintenance of cellular redox homeostasis. Modification of this locus may affect the accumulation of reactive oxygen species and tolerance to ozone-induced oxidative stress. The line is intended for research on the relationship between SHEER-mediated stress signalling, oxidative-stress responses, ozone sensitivity and barley performance.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12110-5 Organism Hordeum vulgare (Barley, HORVU)Crops
EN
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Spring barley with altered stomatal function and ozone sensitivity (hv_ht1)| Usovsko | Changes in physiology and/or production
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Spring barley with altered stomatal function and ozone sensitivity (hv_ost1)| Usovsko | Ozone sensitivity
Sm13-cas9-ec64420_Barley
EN
- Gene editing (e.g. CRISPR-Cas, etc.)
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-301041-1 Stress-induced heme receptor | Hordeum vulgare (Barley, HORVU)Protein coding sequence | Changes in physiology and/or production
Information on the modified DNA sequence
The hv_sheer line was developed through CRISPR/Cas-mediated targeted mutagenesis of the barley SHEER gene. The gene is approximately 6 kb long and comprises eight exons. It encodes the Stress-Induced HEmE Receptor (SHEER), a heme-binding protein implicated in stress signalling and the maintenance of cellular redox homeostasis.
CRISPR/Cas-mediated mutagenesis using two guide RNAs produced a 47-bp deletion in the first exon of the gene. Because the deletion is not divisible by three, it is expected to alter the reading frame and functionally inactivate the target gene. Functional characterization of Hvsheer knockout lines found increased accumulation of reactive oxygen species under ozone-induced oxidative stress compared with wild-type barley, indicating that SHEER contributes to the regulation of redox homeostasis and oxidative-stress tolerance. The progeny selected for the field trial does not contain transgenic sequences originating from the expression vector used to induce the targeted mutation.
The hv_sheer line was developed through CRISPR/Cas-mediated targeted mutagenesis of the barley SHEER gene. The gene is approximately 6 kb long and comprises eight exons. It encodes the Stress-Induced HEmE Receptor (SHEER), a heme-binding protein implicated in stress signalling and the maintenance of cellular redox homeostasis.
CRISPR/Cas-mediated mutagenesis using two guide RNAs produced a 47-bp deletion in the first exon of the gene. Because the deletion is not divisible by three, it is expected to alter the reading frame and functionally inactivate the target gene. Functional characterization of Hvsheer knockout lines found increased accumulation of reactive oxygen species under ozone-induced oxidative stress compared with wild-type barley, indicating that SHEER contributes to the regulation of redox homeostasis and oxidative-stress tolerance. The progeny selected for the field trial does not contain transgenic sequences originating from the expression vector used to induce the targeted mutation.
EN
- Research
The hv_sheer allele can be identified by PCR using primers F_hv_sheer and R_hv_sheer. The wild-type allele produces a 481-bp amplicon, whereas the modified allele produces a 434-bp amplicon owing to the 47-bp deletion. Sequencing of the amplified region can be used to confirm the deletion and determine the zygosity of the plants.
EN
