New Leaf Plus Potatoes showed that the nutritional properties of the potato tubers were substantially equivalent to those of the conventional Russet Burbank and similar white potato varieties as shown by the analyses of key nutrients including proximates (ash, moisture, calories), total solids, sugars, vitamin c, soluble protein and natural glycoalkaloid toxicants. The results of compositional analysis showed that genetic improvement through the Agrobacterium mediated transformation of New Leaf Plus Potatoes did not alter nutrient composition from that of conventionally bred potatoes. New Leaf Plus Potatoes are as safe for consumption as conventional potatoes. There are no differences in taste or suitability for processing for New Leaf Plus Potato and the conventional potato. These imply that transformed tubers are substantially equivalent to conventional potatoes.There is a long history of human exposure to the PLRV replicase protein through the consumption of PLRV-infected potatoes, without any reported adverse effects. There is no significant similarity between the PLRV replicase and any known protein toxins. On the basis of this evidence, animal studies of the toxicity of the PLRV replicase protein are considered to be unnecessary and it is concluded that the PLRV replicase protein, as expressed in the New Leaf® Plus potatoes, is non-toxic to humans.