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Genetic element
(GENE)
last updated: 30 Sep 2015
Beta-1,3-1,4-glucanase gene
EN
-
Endo-1,3(4)-b-glucanaseEN
CS-B-1,3(4)-glucanase-SYNTH
EN
Protein coding sequence
Yes
Beta-1,3-1,4-glucanase
EN
This thermostable Beta-1,3-1,4-glucanase is the chimeric product resulting from the recombination of two Beta-1,3-1,4-glucanase from Bacillus amyloliquefaciens and Bacillus macerans.
The resulting protein depolymerises glucans in the aleurone layer and the endosperm during caryopsis germination.
EN
The resulting protein depolymerises glucans in the aleurone layer and the endosperm during caryopsis germination.
The gene introduced into barley was cloned by intragenic recombination of two (1,3-1,4)-β-glucanases from Bacillus amyloliquefaciens and Bacillus macerans. The enzyme is composed of amino acids 1-12 of the glucanase from Bacillus amyloliquefaciens and amino acids 13-214 of glucanase from Bacillus macerans. Tyrosin at position 13 was deleted. The nucleotide sequence was codon-optimized to ensure functionality of the microbial gene in plants by raising the GC content to 63%.
EN
- The production of recombinant proteins in transgenic barley grains [ English ]
- The production of recombinant proteins in transgenic barley grains [ German ]
- Determinants for the enhanced thermostability of hybrid (1,3-1,4)-β-glucanases [ English ]
- Determinants for the enhanced thermostability of hybrid (1,3-1,4)-β-glucanases [ German ]
- Hybrid Bacillus endo-(1,3-1,4)-β-glucanases: Construction of recombinant genes and molecular properties of the gene products. [ English ]
- Hybrid Bacillus endo-(1,3-1,4)-β-glucanases: Construction of recombinant genes and molecular properties of the gene products. [ German ]
- Bacillus amyloliquefaciens - BCH [ English ]
- Paenibacillus macerans - BCH [ English ]
- Endo-1,3(4)-b-glucanase - Wikipedia [ English ]
| Record type | Field | Record(s) | |
|---|---|---|---|
| Living Modified Organism | Introduced or modified genetic element(s) | 1 | |
| Living Modified Organism | Genetic elements construct | 1 | |
