5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium) | BCH-GENE-SCBD-14979 | Genetic element | Biosafety Clearing-House

Genetic element (GENE)
published: 09 Jun 2006 last updated: 12 Feb 2021
General information
5-enolpyruvylshikimate-3-phosphate synthase gene
  • CP4-epsps
  • aroA
  • 3-phosphoshikimate 1-carboxyvinyltransferase
Protein coding sequence
Donor organism
Agrobacterium tumefaciens strain CP4
Characteristics of the protein coding sequence
5-enolpyruvulshikimate-3-phosphate synthase
The cp4 epsps gene was isolated from the CP4 strain of the common soil bacterium Agrobacterium tumefaciens. The enzyme encoded by the sequence is a version of EPSPS that is highly tolerant to inhibition by glyphosate and therefore leads to increased tolerance to glyphosate-containing herbicides. The following reaction is catalyzed by EPSPS:

3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate

Glyphosate specifically binds to and inactivates the enzyme EPSPS, which is part of an important plant biochemical pathway called the shikimate pathway. The shikimate pathway is involved in the biosynthesis of the aromatic amino acids tyrosine, phenylalanine and tryptophan, as well as other aromatic compounds. When plants are treated with glyphosate herbicides they cannot produce the aromatic amino acids that are essential to their survival and, therefore, die.

LMOs containing the glyphosate-tolerant epsps gene allow farmers to use glyphosate-containing herbicides for weed control, which will kill the weeds but not the LMO.
Additional Information
Records referencing this document Show in search
Record type Field Record(s)
Living Modified Organism Introduced or modified genetic element(s) 218
Living Modified Organism Genetic elements construct 218
Laboratory for detection and identification of LMOs Genetic element(s) detectable by the laboratory 16