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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Potato modified for decreased Stomata Density
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Lines: 1, 2, 7, 9, 11
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Organization:Max-Planck-Institute of Molecular Plant Physiology ()Wissenschaftspark Golm Am Mühlenberg 1 14476 Potsdam - Golm Germany,
,Phone:Fax:Email:Website:
This transgenic potato exhibits a reduced amount of stomata (reduced by up to 55% compared to wild-type) by over expressing the SDD1 protein which decreases guard cell density This results in increased leaf roll at high light intensities and decreased tuber yield.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12106-6 Organism Solanum tuberosum (Potato, SOLTU)Crops
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Potato modified for increased stomata density| Max Planck Institute of Molecular Plant Physiology(MPIMP) | Resistance to antibiotics (Kanamycin, Neomycin), Tolerance to abiotic stress (Drought)
pBinARHyg-AtSDD1
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100292-5 Hygromycin B phosphotransferase gene | Streptomyces hygroscopicus (STRHY)Protein coding sequence | Resistance to antibiotics (Hygromycin)
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BCH-GENE-SCBD-48458-4 Stomatal density and distribution 1 gene | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Protein coding sequence | Tolerance to abiotic stress (Drought)
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-100271-5 Octopine Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
In order to decrease guard cell density, a construct (pBinARHyg-AtSDD1) was transferred that causes the potato plants to overexpress the SDD1 protein from Arabidopsis thaliana. AtSDD1 is put under the control of the CaMV 35S promoter and the ocs terminator. An hph gene cassette consisting of the nos promoter, the hph gene, the polyadenylation signal of gene 7 from plasmid pTiAch5 from Agrobacterium tumefaciens, and the nos terminator was used as a selection marker system.
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- Research
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- Summary Notification - European Commission Joint Notification Centre [ English ]
- A subtilisin-like serine protease involved in the regulation of stomatal density and distribution in Arabidopsis thaliana [ English ]
- The Subtilisin-Like Serine Protease SDD1 Mediates Cell-to-Cell Signaling during Arabidopsis Stomatal Development [ English ]
| Record type | Field | Record(s) | |
|---|---|---|---|
| Country's Decision or any other Communication | LMO identification | 1 | |
| Living Modified Organism | Related LMO(s) | 2 | |
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 1 | |
