BCS-GHØØ4-7 - Herbicide-tolerant, insect-resistant cotton | BCH-LMO-SCBD-101018 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 12 Feb 2014
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Herbicide-tolerant, insect-resistant cotton
EN
T304-40
Yes
BCS-GHØØ4-7
LM cotton line, T304-40, is protected against feeding damage by Lepidopteran insect larvae, and is also tolerant to herbicides containing glufosinate ammonium. Insect protection is conferred by expression of a modified Cry1Ab protein from Bacillus thuringiensis and herbicide tolerance is conferred by expression of phosphinothricin acetyltransferase (PAT) from Streptomyces hygroscopicus.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
Characteristics of the modification process
pTDL008 derived from pGSV20
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)
    Protein coding sequence | Resistance to herbicides (Glufosinate)
  • BCH-GENE-SCBD-14985-12 Cry1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-GENE-SCBD-101021-3 Ps7s7 | Subterranean clover stunt virus (SCSV, Subterranean clover stunt virus)
    Promoter
  • BCH-GENE-SCBD-101025-5 NADP-malic enzyme 1 gene 3'UTR and terminator | Flaveria bidentis (Coastal plain yellowtops, FLABI)
    Terminator
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-101416-6 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
  • BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
  • BCH-GENE-SCBD-104947-3 5'e1 Leader | Oryza sativa (Rice, ORYSA)
    Leader
The modified cry1Ab gene1 has been derived from a gene (Genbank accession No. X04698 - first cloned and characterised by Höfte et al (1986)) which, under the latest nomenclature system, is now known as cry1Ab5 (Bacillus thuringiensis toxin nomenclature,database available online at http://www.lifesci.sussex.ac.uk/home/Neil_Crickmore/Bt/toxins2.html).

Sequencing of the 9056 bp inserted transgenic construct and Southern blot analysis revealed an almost full copy of the T-DNA construct (with an incomplete 3`me1 terminator) was inserted into the T304-40 LM cotton line in addition to:

• a partial 3’me1 terminator;
• a partial copy of the cry1Ab gene cassette, with a trunckated Ps7s7 promoter, in a tail-to-tail orientation, and
• a partial copy of the bar gene cassette in which the nos terminator is truncated.

As a result of the transformation event, four new junctions have been created (see Figure 4 in the attached file below), two being located at the 5’ and 3’ ends of the insert and two being located within the insert as a result of the rearrangement. Southern blot analysis revealed no vector backbone sequences in cotton line T304-40.

For further description of the T304-40 LM cotton, see the safety assessment document attached below.
EN
LMO characteristics
EN
  • Food
  • Feed
  • Fiber/textile
  • Other (Industrial)
Detection method(s)
EN
Additional Information
EN
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