DAS-Ø15Ø7-1 × MON-ØØ81Ø-6 - Maize modified for insect resistance and herbicide tolerance | BCH-LMO-SCBD-103256 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 24 Jul 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Maize modified for insect resistance and herbicide tolerance
EN
TC1507 x MON810
Yes
DAS-Ø15Ø7-1 × MON-ØØ81Ø-6
The stacked maize line was produced through conventional breeding technique of crossing the Herculex I corn event TC1507 with MON810 maize. The cross is insect-resistant maize, tolerant to the herbicide glufosinate ammonium.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)
    Crops
  • BCH-LMO-SCBD-14841-13 Living Modified Organism DAS-Ø15Ø7-1 - Herculex™ I maize
    Dow AgroSciences, Pioneer Hi-Bred International Inc. | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths)), Resistance to herbicides (Glufosinate)
  • BCH-LMO-SCBD-14750-19 Living Modified Organism MON-ØØ81Ø-6 - YieldGard™ maize
    Monsanto | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), European corn borer (Ostrinia nubilalis))
EN
Characteristics of the modification process
PHI8999A, PV-ZMBK07 and PV-ZMGT10
EN
  • Cross breeding
 
0.610 kb
 
 
0.800 kb
 
 
3.460 kb
 
 
1.980 kb
 
 
1.820 kb
 
 
0.720 kb
 
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-14985-12 Cry1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-100359-7 Hsp70 intron | Zea mays (Maize, Corn, MAIZE)
    Intron
  • BCH-GENE-SCBD-14987-8 Cry1F | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-GENE-SCBD-15002-4 Phosphinothricin N-acetyltransferase gene | Streptomyces viridochromogenes (STRVR)
    Protein coding sequence | Resistance to herbicides (Glufosinate)
  • BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)
    Promoter
  • BCH-GENE-SCBD-100363-5 ORF25 PolyA Terminator sequence | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)
    Terminator
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
DNA insert from MON810, vectors PV-ZMBK07 and PV-ZMGT10:

MON810 contains a truncated portion of a synthetic form of the cry1Ab gene from Bacillus thuringiensis subsp. Two constructs PV-ZMBK07 and PV-ZMGT10 have been used for transformation, but molecular analyses showed that MON810 does not contain any element from PV-ZMGT10 construct and only the elements from construct PV-ZMBK07 have been integrated into its genome.

DNA insert from TC1507, vector PHI8999A: 

DNA fragment PHI8999A contains two adjacent plant gene expression cassettes for the cry1F and pat genes

For additional information on this LMO, please refer to the records of the parental LMOs.
EN
LMO characteristics
EN
  • Food
  • Feed
Detection method(s)
MON810 contains one integrated DNA consisting of P-e35S, I-Hsp70 and cry1Ab. The terminator of the nopaline synthase (nos) gene was lost due to a truncation at the 3' end of the gene cassette during genome integration and is, therefore, not present in MON810.

Southern analysis using a cry1F probe carried out by the European Food Safety Authority revealed the presence of two cry1F inserts. The first represented the intact gene from the expression cassette. The second insert was a truncated cry1F  fragment of 335bp, which is located at the 5' end of the insertion locus. In addition, analysis of the sequences adjacent to the insert of fragment PHI8999A revealed DNA fragments that correspond to small segments from PHI8999A, including incomplete sequences from the pat gene, the maize ubiquitin promoter and the terminator from Agrobacterium. Furthermore, different fragments of chloroplast DNA and a number of sequences with similarity to retrotransposons are also present in the border region of the insert.
EN
Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
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