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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Enlist™ Maize
EN
DAS-40278-9
Yes
DAS-4Ø278-9
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Person:Dow AgroSciences GmbHTruderinger Straße 15München, Bayern
81677, GermanyPhone: + 49 89 - 4 55 33 - 0,Fax: + 49 89 - 4 55 33 - 111,Email: DowAgroSciencesD@dow.com,Website: http://www.dowagro.com/de,Related OrganizationDow AgroSciences GmbH ()Private sector (business and industry)Truderinger Straße 15München, Bayern
81677, GermanyPhone: + 49 89 - 4 55 33 - 0,Fax: + 49 89 - 4 55 33 - 111,Email: DowAgroSciencesD@dow.com,Website: http://www.dowagro.com/de,
The maize DAS-4Ø278-9 was modified for tolerance to the herbicides 2,4-dichlorophenoxyacetic acid (2,4-D) and aryloxyphenoxypropionate (AOPP) acetyl coenzyme A carboxylase (ACCase) inhibitors. To achieve herbicide tolerance, the maize expresses an optimised coding sequence for aryloxyalkanoate dioxygenase from Sphingobium herbicidovoran.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)Crops
EN
pDAS1740
EN
- Direct DNA transfer
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1.166 kb
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1.991 kb
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0.891 kb
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0.365 kb
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1.166 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-104795-4 RB7 matrix attachment region | Nicotiana tabacum (Tobacco, TOBAC )Enhancer
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
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BCH-GENE-SCBD-104812-3 Aryloxyalkanoate dioxygenase gene | Sphingobium herbicidovorans (SPHHE)Protein coding sequence | Resistance to herbicides,Tolerance to 2,4-Dichlorophenoxyacetic acid,Tolerance to aryloxyphenoxypropionate
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BCH-GENE-SCBD-104813-4 Per5 3' Untranslated Region | Zea mays (Maize, Corn, MAIZE)Terminator
Information about the inserted DNA sequences
The DNA insert from maize DAS-4Ø278-9 comprised a genetic cassette expressing the gene encoding aryloxyalkanoate dioxygenase (aad1) from Sphingobium herbicidovoran using the Whiskers mediated transformation method.
The aad1 gene is regulated by the Zea mays polyubiquitin gene promoter and the peroxidase 5 3' Untranslated Region. The aad1 coding sequence was modified for plant-optimized expression. High levels of transcription are expected due to the strong constitutive promoter. Nicotiana tabacum RB7 matrix attachment 5' and 3' regions were also inserted (MAR) to enhance structural anchoring of chromosomes to the cell's nuclear matrix.
The DNA insert from maize DAS-4Ø278-9 comprised a genetic cassette expressing the gene encoding aryloxyalkanoate dioxygenase (aad1) from Sphingobium herbicidovoran using the Whiskers mediated transformation method.
The aad1 gene is regulated by the Zea mays polyubiquitin gene promoter and the peroxidase 5 3' Untranslated Region. The aad1 coding sequence was modified for plant-optimized expression. High levels of transcription are expected due to the strong constitutive promoter. Nicotiana tabacum RB7 matrix attachment 5' and 3' regions were also inserted (MAR) to enhance structural anchoring of chromosomes to the cell's nuclear matrix.
Note
- Southern blot analysis indicated that a single complete copy of the transformation cassette was stably integrated into the host genome at a single locus and that there was no integration of segments from the vector backbone.
EN
- Feed
- Food
EN
EN
- EUginius: DAS40278 [URL] [ English ]
- OECD Biotrack database: DAS-40278-9 [URL] [ English ]
- ISAAA GM Approval database: DAS40278 [URL] [ English ]
- USDA APHIS: DOW Petition for Nonregulated Status of DAS-40278-9 Corn [PDF] [ English ]
- FSANZ Safety Assessment. Herbicide-tolerant corn line DAS-40278-9 (A1042) [PDF] ( Food Standards Australia New Zealand ) [ English ]
- Patent (2011). Événement das-40278-9 d'aad-1, lignées transgéniques de maïs connexes et identification spécifique d'événement de celui-ci [PDF] ( WO2011022469A2, WIPO (PCT) ) [ French ]
- Patent (2018). Aad-1 event das-40278-9, related transgenic corn lines, and event-specific identification thereof [PDF] [ English ]
- Patent (2013). AAD-1 event DAS-40278-9, related transgenic corn lines, event-specific identification thereof, and methods of weed control involving AAD-1 [PDF] ( US8598413 ) [ English ]
- Regul Toxicol Pharmacol (2011). Performance of broiler chickens fed event DAS-40278-9 maize containing the aryloxyalkanoate dioxygenase-1 protein [DOI] [ English ]
- GM Crops (2011). Compositional safety of event DAS-40278-9 (AAD-1) herbicide-tolerant maize [PDF] [ English ]
- GM Crops & Food (2018). Safety evaluation of genetically modified DAS-40278-9 maize in a subchronic rodent feeding study [DOI] [ English ]
- Agricultural and Food Chemistry (2016). Metabolism and Residues of 2,4-Dichlorophenoxyacetic Acid in DAS-40278-9 Maize (Zea mays) Transformed with Aryloxyalkanoate Dioxygenase-1 Gene [DOI] [ English ]
- CFIA Decision document 2012-92: Determination of the safety of Dow AgroSciences Canada Inc.'s corn (Zea mays L.) event DAS-40278-9 [URL] [ English ]
- FAO GM Foods Platform: DAS-40278-9 [URL] [ English ]
| Record type | Field | Record(s) | |
|---|---|---|---|
| Living Modified Organism | Recipient Organism or Parental Organisms | 40 | |
| Living Modified Organism | Related LMO(s) | 1 | |
| Country's Decision or any other Communication | LMO identification | 19 | |
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 19 | |
| Laboratory for detection and identification of LMOs | LMO(s) detectable by the laboratory | 8 | |
