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Living Modified Organism
(LMO)
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VECTORMUNE® HVT-IBD
EN
HVT-IBD
No
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Person:Dr Alexis Henry Gaetan GouxPresident of CIBio,Rua Manuel Joaquim Filho, 303 Pulínia - SPPaulínia, São Paulo
13140-000, BrazilPhone: 551938337700,Fax: 55193833-7722,Email: alexisgoux@ceva.com,Website: http://www.ceva.com.br/,Related OrganizationCeva Saúde Animal Ltda (CEVA)Private sector (business and industry)Rua Manuel Joaquim Filho, 303 Pulínia - SPPaulínia, São Paulo
13140-000, BrazilPhone: 551938337700,Fax: 55193833-7722,Email: alexisgoux@ceva.com,Website: http://www.ceva.com.br/,
VECTORMUNE® IBD is a chicken vaccine that contains a genetically engineered Marek’s Disease virus of serotype 3 (turkey Herpesvirus or HVT) expressing Infectious Bursal Disease key protective antigens, therefore providing immunity against IBD.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-105225-3 Organism Meleagrid alphaherpesvirus 1 (Turkey herpesvirus, Meleagrid herpesvirus 1)Viruses
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pUC18
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- Other (Homologous Recombination)
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-105217-2 Beta-actin gene promoter | Gallus gallus (Chicken, CHICK)Promoter
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BCH-GENE-SCBD-105223-1 Viral Protein 2 gene | Infectious bursal disease virus (Gumboro virus)Protein coding sequence | Production of medical or pharmaceutical compounds (human or animal) (Vaccines)
A 2.9 kb region of the HVT genome was inserted into the pUC18 vector. This fragment contained the incomplete open reading frame (ORF) of UL44 and the complete ORFs of UL45 and UL46 from the HVT genome.
The chicken beta-actin promoter and transcription termination sequences from SV40 and UL46 were inserted into the HVT genoming DNA fragment.
The VP2 gene from the Delaware Variant of the IBDV was amplified from viral RNA using RT-PCR and inserted in to the pUC18 vector in between the beta-actin promoter and termination sequences from SV40 and UL46.
This construct and the the parental HVT strain were co-transformed into chicken embryo fibroblasts and incubated under conditions that favour homologous recombination.
Viral particles were cultivated from the chicken embryo fibroblasts and plaques were expanded and screened and selected for the expression of the VP2 gene.
EN
The chicken beta-actin promoter and transcription termination sequences from SV40 and UL46 were inserted into the HVT genoming DNA fragment.
The VP2 gene from the Delaware Variant of the IBDV was amplified from viral RNA using RT-PCR and inserted in to the pUC18 vector in between the beta-actin promoter and termination sequences from SV40 and UL46.
This construct and the the parental HVT strain were co-transformed into chicken embryo fibroblasts and incubated under conditions that favour homologous recombination.
Viral particles were cultivated from the chicken embryo fibroblasts and plaques were expanded and screened and selected for the expression of the VP2 gene.
EN
- Vaccine
EN
EN
- VECTORMUNE® HVT-IBD - CTNBio [ English ]
- Vectormune® IBD - Vectormune [ English ]
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