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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Reduced Lignin Alfalfa
EN
KK179
Yes
MON-ØØ179-5
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Organization:Monsanto ()800 North Lindbergh Blvd.St. Louis, MO
63167, United States of AmericaPhone: + 1 314 694-1000,Fax: +1 314 694-3080,Email:Website: http://www.monsanto.com,
Alfalfa KK179 was developed to have reduced levels of guaiacyl lignin (G), a major subunit component of total lignin, compared to conventional alfalfa at the same stage of growth.
This reduction in G lignin leads to reduced accumulation of total lignin in alfalfa through the suppression of caffeoyl CoA 3-O-methyltransferase (CCOMT), a key enzyme in the lignin biosynthetic pathway.
EN
This reduction in G lignin leads to reduced accumulation of total lignin in alfalfa through the suppression of caffeoyl CoA 3-O-methyltransferase (CCOMT), a key enzyme in the lignin biosynthetic pathway.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12100-4 Organism Medicago sativa (Alfalfa, Lucerne, MEDSV)Crops
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PV-MSPQ12633
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-105595-1 Pal2 gene Promoter | Phaseolus vulgaris (String bean, French bean, Kidney bean, Common Bean , PHAVU)Promoter
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BCH-GENE-SCBD-105596-1 Caffeoyl CoA 3-O-methyltransferase gene | Medicago sativa (Alfalfa, Lucerne, MEDSV)Protein coding sequence | Changes in quality and/or metabolite content (Lignin)
The transformation cassette in KK179 consists of inverted repeat DNA segments of the CCOMT gene derived from alfalfa. The inverted repeat sequence produces double-stranded RNA (dsRNA) which suppresses endogenous CCOMT gene expression via the RNA interference (RNAi) pathway. Suppression of the CCOMT gene expression leads to lower CCOMT protein expression resulting in reduced synthesis of G lignin subunit.
The PV-MSPQ12633 vector contained two transformation cassettes. the first consisting of the CCOMT inverted repeat sequence and the other containing the nptII coding sequence.
Southern blot analysis showed that only a single copy of the CCOMT transformation cassette was transformed into the alfalfa genome. No plasmid vector backbone sequences are present in KK179 and no DNA sequences from the nptII transformation cassette were detected.
EN
The PV-MSPQ12633 vector contained two transformation cassettes. the first consisting of the CCOMT inverted repeat sequence and the other containing the nptII coding sequence.
Southern blot analysis showed that only a single copy of the CCOMT transformation cassette was transformed into the alfalfa genome. No plasmid vector backbone sequences are present in KK179 and no DNA sequences from the nptII transformation cassette were detected.
EN
- Feed
- MON-ØØ179-5 - CropLife International Detection Methods Database ( CropLife ) [ English ]
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- MON-ØØ179-5 - APHIS [ English ]
- MON-ØØ179-5 - FDA [ English ]
- MON-ØØ179-5 - FSANZ [ English ]
- MON-ØØ179-5 - Japan BCH [ English ]
- MON-ØØ179-5 - ISAAA [ English ]
| Record type | Field | Record(s) | |
|---|---|---|---|
| Country's Decision or any other Communication | LMO identification | 6 | |
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 5 | |
| Living Modified Organism | Recipient Organism or Parental Organisms | 1 | |
