MON-887Ø1-3 - Dicamba- and Glufosinate-tolerant cotton | BCH-LMO-SCBD-105602 | Living Modified Organism | Biosafety Clearing-House

Living Modified Organism (LMO)
Decisions on the LMO Risk Assessments  
published: 11 Jun 2014 last updated: 20 Jan 2023
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Dicamba- and Glufosinate-tolerant cotton
The cotton (Gossypium hirsutum) was modified for tolerance to the herbicides dicamba (2-methoxy-3,6-dichlorobenzoic acid) and glufosinate. For tolerance to dicamba, the cotton expresses Stenotrophomonas maltophilia dicamba monooxygenase, which inactivates the herbicide via oxidative demethylation. For tolerance to glufosinate, the cotton expresses Streptomyces hygroscopicus phosphinothricin N-acetyltransferase, which inactivate the herbicide via acetylation. 
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Variety Coker 130
  • Cotton transformation events tolerant to the herbicides Dicamba and Glufosinate
    | Resistance to herbicide: Dicamba, Resistance to herbicides (Glufosinate)
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-104662-3 PCSV Promoter | Peanut chlorotic streak virus (PCSV, PClSV)
  • BCH-GENE-SCBD-104664-2 TEV 5' Untranslated Region | Tobacco etch virus (TEV)
  • BCH-GENE-SCBD-100728-3 Dicamba monooxygenase gene | Stenotrophomonas maltophilia (S. maltophilia, Stenotrophomonas)
    Protein coding sequence | Resistance to herbicides
  • BCH-GENE-SCBD-103901-2 HSP 70 5' untranslated leader sequence | Petunia hybrida (Petunia, PETHY)
  • BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)
    Protein coding sequence | Resistance to herbicides (Glufosinate)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
  • BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Transit signal
  • BCH-GENE-SCBD-105600-1 E6 gene terminator | Gossypium barbadense (Sea-island cotton, Egyptian cotton, GOSBA)
  • BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)
The modified cotton contains two gene cassettes: Stenotrophomonas maltophilia dicamba monooxygenase (dmo) and Streptomyces hygroscopicus phosphinothricin N-acetyltransferase (bar).

The dmo coding sequence is under control of a Peanut chlorotic streak virus promoter and Gossypium barbadense E6 terminator. A Tobacco etch virus 5' untranslated region was added between the promoter and dmo coding sequence to enhance transcription of dmo.

The bar coding sequence is under control of a Cauliflower mosaic virus 35S enhanced promoter and an Agrobacterium tumefaciens nopaline synthases terminator. A Petunia hybrida heat shock protein 70 leader was added between the viral promoter and the bar coding sequence to enhance expression of bar.

Southern Blot and sequence analysis indicated that the transformation cassette was inserted into the MON88701 genome at a single intact locus containing all the genetic elements without the integration of vector backbone sequences. 
LMO characteristics
  • Feed
  • Fiber/textile
  • Food
Detection method(s)
Additional Information