SYN-ØØØH2-5 - Soy modified for tolerance to Mesotrione and Glufosinate | BCH-LMO-SCBD-105619 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 12 Jun 2014
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Soy modified for tolerance to Mesotrione and Glufosinate
Soybean SYHT0H2, has been developed to be tolerant to two herbicides with different modes of action, namely glufosinate-ammonium and mesotrione.

Tolerance to glufosinate is achieved through expression of the enzyme phosphinothricin acetyltransferase (PAT). Tolerance to mesotrione is achieved through expression of the p-hydroxyphenylpyruvate dioxygenase (AvHPPD-03) protein .
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Cultivar: Jack
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
The enzyme phosphinothricin acetyltransferase in the two different expression cassettes are encoded by two similar pat genes pat-03-01 which is regulated by the 35s promoter and pat-03-02 which is regulated by the CMP promoter. Both coding sequences encode identical PAT proteins.

The avhppd-03 was codon optimised for enhanced expression.

Southern blot and sequencing analysis indicated that SYHT0H2 contains, at a single locus, a single copy of avhppd-03, four copies of the pat gene, one copy of the avhppd-03 promoter complex, two copies of the 35s promoter, two copies of the CMP promoter, two copies of the TMV enhancer and five copies of the nos terminator sequences
LMO characteristics
  • Food
  • Feed
Detection method(s)
Additional Information
Records referencing this document Show in search
Record type Field Record(s)