Barley modified for tolerance to drought and nutrient deficiencies | BCH-LMO-SCBD-108704 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 10 Sep 2015
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Barley modified for tolerance to drought and nutrient deficiencies
EN
bGLU:CKX1
No
The bGLU:CKX1 barley line has been genetically modified by inserting the Arabidopsis thaliana cytokinin dehydrogenase 1 gene under the control of the root-specific β-glucosidase promoter from maize.

Cytokinin dehydrogenases catalyze the irreversible degradation of cytokinins in a single enzymatic step by oxidative side chain cleavage. Cytokinins, which are chemically N6-substituted purine derivatives, are a class of plant hormones that regulate cell division as well as a large number of developmental events in plants. An important trait regulated by cytokinin is the size of the root system.

A reduced cytokinin status in plants (including barley) causes an enhanced root system which might render plants more tolerant to drought and nutrient deficiencies in the soil. Crop yield is often limited by the availability of water and soil-derived mineral nutrients. A larger root system may enable plants to get better access to water and nutrient resources, therefore to better cope with adverse environmental conditions.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
cv. Golden Promise
EN
  • Barley modified for tolerance to drought and nutrient deficiencies
    | Palacky University in Olomouc | Resistance to antibiotics (Hygromycin), Selectable marker genes and reporter genes, Tolerance to abiotic stress (Drought)
  • Barley modified for tolerance to drought and nutrient deficiencies
    | Palacky University in Olomouc | Resistance to antibiotics (Hygromycin), Selectable marker genes and reporter genes, Tolerance to abiotic stress (Drought)
  • Barley modified for tolerance to drought and nutrient deficiencies
    | Palacky University in Olomouc | Resistance to antibiotics (Hygromycin), Selectable marker genes and reporter genes, Tolerance to abiotic stress (Drought)
Characteristics of the modification process
bGLU:CKX1
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-108700-1 Beta-glucosidase gene promoter | Zea mays (Maize, Corn, MAIZE)
    Promoter
  • BCH-GENE-SCBD-108701-2 Cytokinin dehydrogenase 1 gene | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Protein coding sequence | Tolerance to abiotic stress (Drought)
  • BCH-GENE-SCBD-108703-1 TMV 35s promoter | Tobacco mosaic virus (TMV)
    Promoter
  • BCH-GENE-SCBD-14991-8 Hygromycin B phosphotransferase gene | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Hygromycin),Selectable marker genes and reporter genes
The transgenic line shows an up to 8-fold higher CKX enzyme activity in roots as compared to the wild type. In contrast, CKX enzymatic activity in the leaves of transgenic plants is similar to the control plants.

The cytokinin dehydrogenase 1 gene is under the control of the root-specific β-glucosidase promoter from maize.

The Hygromycin B phosphotransferase selection marker is under the control of the TMV 35s promoter.
EN
LMO characteristics
EN
  • Research
Detection method(s)
EN
Additional Information
EN
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