Barley modified for tolerance to drought and nutrient deficiencies | BCH-LMO-SCBD-108710 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 02 Sep 2015
Living Modified Organism identity
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Barley modified for tolerance to drought and nutrient deficiencies
EN
CKX1:RNAi
No
The CKX1:RNAi barley line has been genetically modified by inserting the a copy of the native Hordeum vulgare cytokinin dehydrogenase 1 gene under the control of the constitutive ubiquitin gene promoter from maize.

Cytokinin dehydrogenases catalyze the irreversible degradation of cytokinins in a single enzymatic step by oxidative side chain cleavage. Cytokinins, chemically N6-substituted purine derivatives, are a class of plant hormones that regulate cell division as well as a large number of developmental events in plants.

Beside the others, cytokinins influence grain filling by regulation of the cell-wall invertase, the important enzyme in a polysacharides metabolism.

Increased cytokinin levels in the grains might enhance the process of grain filling and lead to the production of bigger grains. As the HvCKX1 gene is naturally expressed in the young barley roots and grains, the constitutive expression should not influence the other parts of the plant.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
cv. Golden Promise
EN
  • Barley modified for tolerance to drought and nutrient deficiencies
    | Palacky University in Olomouc | Resistance to antibiotics (Hygromycin), Selectable marker genes and reporter genes, Tolerance to abiotic stress (Drought)
  • Barley modified for tolerance to drought and nutrient deficiencies
    | Palacky University in Olomouc | Resistance to antibiotics (Hygromycin), Selectable marker genes and reporter genes, Tolerance to abiotic stress (Drought)
  • Barley modified for tolerance to drought and nutrient deficiencies
    | Palacky University in Olomouc | Resistance to antibiotics (Hygromycin), Selectable marker genes and reporter genes, Tolerance to abiotic stress (Drought)
Characteristics of the modification process
CKX1:RNAi
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-108709-1 Cytokinin dehydrogenase 1 gene | Hordeum vulgare (Barley, HORVU)
    Protein coding sequence | Tolerance to abiotic stress (Drought)
  • BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)
    Promoter
  • BCH-GENE-SCBD-14991-8 Hygromycin B phosphotransferase gene | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Hygromycin),Selectable marker genes and reporter genes
The transgenic line showed an up 4-fold lower CKX activity in the barley grains compared to the wild type plants. The total yield from CKX1:RNAi barley was 20% higher than the yield of wild type barley.

The cytokinin dehydrogenase 1 silencing cassette is under the control of the constitutive ubiquitin gene promoter from maize.

The Hygromycin B phosphotransferase selection marker is under the control of the promoter region from Zea mays ubiquitin gene.
EN
LMO characteristics
EN
Detection method(s)
EN
Additional Information
EN
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