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Living Modified Organism
(LMO)
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Rubber tree modified to express Human Protamine 1
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HT4
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Person:Dr. Sunderasan ElumalaiResearch Officer, Genetic Transformation and Tissue Culture Programme, Production Advancement DivisionGenetic Transformation and Tissue Culture Programme, Production Advancement Division, Malaysian Rubber Board,Sungai Buloh, Selangor
47000, MalaysiaPhone: +603-61459599,Fax: +603-61565251,Email: sunderasan@lgm.gov.my,Website:Related OrganizationMalaysian Rubber Board (LGM)Academic or research instituteGenetic Transformation and Tissue Culture Programme, Production Advancement Division, Malaysian Rubber Board,Sungai Buloh, Selangor
47000, MalaysiaPhone: +603-61459599,Fax: +603-61565251,Email: sunderasan@lgm.gov.my,Website:
Hevea brasiliensis was modified to express the human protamine 1 (HP1), which is a therapeutic protein which is commonly used to neutralize the anticoagulant effects of heparin during cardiovascular surgery. Expression of the HP1 gene in Hevea brasiliensis allows for the commercial production of this protein for pharmaceutical purposes.
Hevea expressing HP1 also harbours nptII gene that confers resistance to kanamycin, which was incorporated in the growth media to select transformed callus after co-cultivation with Agrobacterium.
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Hevea expressing HP1 also harbours nptII gene that confers resistance to kanamycin, which was incorporated in the growth media to select transformed callus after co-cultivation with Agrobacterium.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-108280-2 Organism Hevea brasiliensis (Rubber tree, Para rubber tree, SiringaTree, Jebe, HEVBR)Trees
Clone GL1
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pGPTV-HP1-HevP
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-108283-1 Protamine 1 coding sequence | Homo sapiens (HUMAN)Protein coding sequence | Production of medical or pharmaceutical compounds (human or animal)
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BCH-GENE-SCBD-108907-1 Hevien gene promoter | Hevea brasiliensis (Rubber tree, Para rubber tree, SiringaTree, Jebe, HEVBR)Promoter
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-103067-9 Transcript 7 gene 3' untranslated region | Agrobacterium tumefaciens (Agrobacterium)Terminator
The four vector constructs were synthesized each containing a variable length of the heaven gene promoter (pGPTV-HP1-HevP1, pGPTV-HP1-HevP2, pGPTV-HP1-HevP3, and pGPTV-HP1-HevP4) each of which was placed upstream of the protamine 1 coding sequence.
The HP1 and nptII genetic elements were placed in an antisense orientation relative to each other within the plasmid.
The inserted genetic material is the region between the left boarder and the right boarder (T-DNA) from the Ti plasmid of Agrobacterium tumefaciens harbouring the above described gene construct.
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The HP1 and nptII genetic elements were placed in an antisense orientation relative to each other within the plasmid.
The inserted genetic material is the region between the left boarder and the right boarder (T-DNA) from the Ti plasmid of Agrobacterium tumefaciens harbouring the above described gene construct.
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- Pharmaceutical
- Research
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