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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Petunia with a modified flower colour
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RL01-17
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Person:MPIPZCarl-von-Linné-Weg 10 50829 KölnKöln,
, GermanyPhone: +49 221 5062-0,Fax: +49 221 5062-674,Email: prag@mpipz.mpg.de,Related OrganizationMax Planck Institute for Plant Breeding Research (MPIPZ)Academic or research instituteCarl-von-Linné-Weg 10 50829 KölnKöln,
, GermanyPhone: +49 221 5062-0,Fax: +49 221 5062-674,Email: prag@mpipz.mpg.de,
Petunia was genetically modified to express the A1 gene of Zea mays which is codes for dihydroflavonol-4-reductase. This reductase converts endogenously accumulated dihydrokaempferol to leukopelargonidin, resulting in the production of the pigment pelargonidin and therefore a salmon red flower colour.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12107-5 Organism Petunia hybrida (Petunia, PETHY)Crops
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p35A1
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- Direct DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100271-5 Octopine Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-111603-2 Dihydroflavonol-4-reductase | Zea mays (Maize, Corn, MAIZE)Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
The A1 gene coding for the dihydroflavonol-4-reductase from Zea mays (CS-dfr-MAIZE) is expressed under the control of the 35S promoter and termination region of the Cauliflower Mosaic Virus (P-35S-CaMV and T-35S-CaMV).
Approximately 200 bp of the native genome were deleted at the insertion site. There is no evidence that this would lead to any functional changes.
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Approximately 200 bp of the native genome were deleted at the insertion site. There is no evidence that this would lead to any functional changes.
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- Research
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