Petunia with a modified flower colour | BCH-LMO-SCBD-111614 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)
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BCH-LMO-SCBD-111614-2   |   PDF   |   Print   |  
Decisions on the LMO Risk Assessments  
last updated: 12 May 2017
Living Modified Organism identity
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Petunia with a modified flower colour
EN
RL01-17
No
Petunia was genetically modified to express the A1 gene of Zea mays which is codes for dihydroflavonol-4-reductase. This reductase converts endogenously accumulated dihydrokaempferol to leukopelargonidin, resulting in the production of the pigment pelargonidin and therefore a salmon red flower colour.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
Characteristics of the modification process
p35A1
EN
  • Direct DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)
    Terminator
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
    Promoter
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-100271-5 Octopine Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-111603-2 Dihydroflavonol-4-reductase | Zea mays (Maize, Corn, MAIZE)
    Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
The A1 gene coding for the dihydroflavonol-4-reductase from Zea mays (CS-dfr-MAIZE) is expressed under the control of the 35S promoter and termination region of the Cauliflower Mosaic Virus (P-35S-CaMV and T-35S-CaMV).
Approximately 200 bp of the native genome were deleted at the insertion site. There is no evidence that this would lead to any functional changes.
EN
LMO characteristics
EN
  • Research
Detection method(s)
EN
Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
Living Modified Organism Related LMO(s) 2
Country's Decision or any other Communication Living modified organism(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1