CTC-91Ø87-6 - Insect resistant sugarcane | BCH-LMO-SCBD-115593 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 29 May 2020
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Insect resistant sugarcane
  • - Person: Dr Wladecir Salles Oliveira | BCH-CON-SCBD-112453-2
    Dr Wladecir Salles Oliveira
    Chairman of the Internal Biosafety Committee,
    Fazenda Santo Antônio s/n, Caixa Postal 162
    Piracicaba, Sao Paulo
    13400-970, Brazil
    Phone: +55 (11) 3429-8111,
    Fax: +55 (11) 3429-8111,
    Related Organization
    CTC - Centro de Tecnologia Canavieira (CTC)
    Regional economic integration organization
    Fazenda Santo Antônio s/n, Caixa Postal 162
    Piracicaba, Sao Paulo
    13400-970, Brazil
    Phone: +55 (11) 3429-8111,
    Fax: +55 (11) 3429-8111,
The sugarcane was modified for resistance to sugarcane borer (Diatraea saccharalis) through the expression of Bacillus thuringiensis Cry1Ac. The sugarcane additionally contains Streptomyces hygroscopicus phosphinothricin N-acetyltransferase gene as a selectable marker.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Sugarcane early variety CTC9001
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Genetic cassettes:
The modified sugarcane contains two gene cassettes: Bacillus thuringiensis cry1Ac and Streptomyces hygroscopicus phosphothricin N-acetyltransferase (pat). Both gene cassettes are under transcriptional control of the Zea mays ubiquitin promoter and the Agrobacterium tumefaciens nopaline synthase terminator.

- The coding sequence for cry1Ac was optimized for expression in sugarcane (sugarcane preferred codons).
- High levels of transcription are expected due to the constitutive nature of the ubiquitin promoter.
LMO characteristics
  • Food
  • Biofuel
Detection method(s)
The Cry1Ac encodes a 615 amino acid protein with an estimated molecular weight of 68 kDa. The insecticidal core of the protein is 52 kDA.

DNA and protein on sugar samples produced from this modified sugarcane were unable to detection Cry1Ac or PAT (below the levels of detection) according Gianotto et al. (2019) (see attached document).
Additional Information
Records referencing this document Show in search
Record type Field Record(s)
Living Modified Organism Related LMO(s) 1