Banana modified for Xanthomonas wilt disease resistance | BCH-LMO-SCBD-115602 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 11 Jun 2020
Living Modified Organism identity
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Banana modified for Xanthomonas wilt disease resistance
EN
HRAP
No
The banana was modified for resistance to Xanthomonas wilt disease, caused by Xanthomonas campestris pv. musacearum through the expression of Capsicum annuum hypersensitive response-assisting protein. The modified banana plants demonstrate more a robust hypersensitive response upon infection with Gram negative bacteria, such as X. campestris, and thus resist  disease related wilting. The modified banana also contains a selectable marker, Ecscherichia coli neomycin phosphotransferase II, for kanamycin selection during transformation.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
  • Banana resistant to Xanthomonas wilt disease
    | International Institute of Tropical Agriculture, Kenya(IITA) | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Bacteria), Resistance to Xanthomonas sp., Selectable marker genes and reporter genes
  • Banana modified for Xanthomonas wilt disease resistance
    | International Institute of Tropical Agriculture, Kenya(IITA) | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Bacteria), Resistance to Xanthomonas sp., Selectable marker genes and reporter genes
Characteristics of the modification process
pBI-HRAP
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-101416-6 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
    Promoter
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-113355-1 Hypersensitive response assisting protein gene | Capsicum annuum (Bell pepper, Sweet pepper, Chili pepper, CAPAN)
    Protein coding sequence | Resistance to diseases and pests
  • BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
Gene expression
Transcription of Escherichia coli neomycin phosphotransferase II is under control of the Agrobacterium tumefaciens nopaline synthase (nos) promoter and terminator.

Transcription of Capsicum annuum hypersensitive response assisting protein (hrap) is under control of the Cauliflower Mosaic Virus 35S promoter and the nos terminator. High levels of transcription are expected due to the constitutive nature of the CaMV promoter.

Notes:
- The transformation vector was derived from the pBI121 vector using a BamHI and SacI restriction digest.
- The coding sequence of hrap was derived from the cDNA clone of the mRNA.
EN
LMO characteristics
EN
  • Food
  • Research
Detection method(s)
Some detection methods can be found in the scientific article detailing the creation of this banana event.
EN
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 1
Living Modified Organism Related LMO(s) 2