FLO-4Ø686-3 - Moonstrike™ carnation | BCH-LMO-SCBD-115776 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 23 Nov 2020
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Moonstrike™ carnation
The modified carnation (Dianthus caryophyllus) was derived from the parental Moonvista™ carnation through vegetative propagation. The Moonstrike™ carnation differs from the parental variety in having a flecked, bi-colour flower colour pattern. The modified carnation contains  Petunia hybrida dihydroxflavonol-4 reductase and Viola sp. flavonoid 3' 5' hydroxylase, which together promoter biosynthesis of delphinidin and anthocyanin pigments. The pigment bioproduction results in petals that are eggplant purple in colour. The modified carnation additionally contains Nicotiania tabacum acetolactate synthase for chlorsulfuron selection during tissue culturing.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-ORGA-SCBD-4954-7 Organism Dianthus caryophyllus (Carnation, DIACA)
  • BCH-LMO-SCBD-14835-11 Living Modified Organism FLO-4Ø685-2 - Moonvista™ carnation
    SUntory Holdings Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Sulfonylurea)
Moonstrike™ is a clone of the carnation variety FLORIGENE Moonvista™.
  • FLO-4Ø62Ø-9 - Moonburst™ carnation
    | SUntory Holdings Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Chlorsulfuron, Sulfonylurea)
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
  • BCH-GENE-SCBD-101901-3 5' untranslated leader of chlorophyll a/b-binding protein | Petunia hybrida (Petunia, PETHY)
  • loading record details...
    BCH-GENE-SCBD-15177-7 Acetohydroxy acid synthase gene | Nicotiana tabacum (Tobacco, TOBAC )
    Protein coding sequence | Resistance to herbicides (Chlorsulfuron, Sulfonylurea)
  • BCH-GENE-SCBD-100390-7 Acetohydroxy acid synthase gene terminator | Nicotiana tabacum (Tobacco, TOBAC )
  • BCH-GENE-SCBD-105798-1 Dihydroflavonol-4-reductase promoter | Petunia hybrida (Petunia, PETHY)
  • loading record details...
    BCH-GENE-SCBD-15009-4 Dihydroflavonol-4-reductase | Petunia hybrida (Petunia, PETHY)
    Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
  • BCH-GENE-SCBD-105799-1 Dihydroflavonol-4-reductase terminator | Petunia hybrida (Petunia, PETHY)
  • BCH-GENE-SCBD-103771-1 Chalcone synthase gene promoter | Antirrhinum majus (Common Snapdragon, Snapdragon)
  • BCH-GENE-SCBD-43793-4 Flavonoid 3', 5'-hydroxylase gene | Viola sp. (Pansy, VIOLA)
    Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
  • loading record details...
    BCH-GENE-SCBD-103772-2 D8 gene terminator | Petunia hybrida (Petunia, PETHY)
Gene expression
Three gene cassettes are present: Nicotiania tabacum acetolactate synthase (ALS; acetohydroxy acid synthase), Petunia hybrida dihydroflavonol-4-reductase (DFR) and Viola sp. flavonoid3', 5'-hydroxylase (F3'5'H).

Transcription of ALS is under control of a Cauliflower mosaic virus (CaMV) 35S promoter and a N. tabacum ALS terminator. A 5' untranslated leader sequence from P. hybrida chlorophyll a/b-binding protein is also present at the 5' end of ALS, but is not expected to be translated. The leader sequence promotes high levels of transcription of ALS.

Transcription of DFR is under control of its endogenous promoter and terminator. The coding sequence contains 6 exons and 5 introns.

Transcription of F3'5'H is under control of an Antirrhinum majus chalcone synthase promoter and a P. hybrida D8 terminator.

- The size of the ALS coding sequence includes the size of the terminator (3.76 kb = size of ALS coding sequence + ALS terminator)
-  The size of the DFR coding sequence represents the size of the full genomic cone (4.96 kb = DFR promoter + DFR coding sequence + DFR terminator)

For more information, kindly refer to the parental record.
LMO characteristics
  • Ornamental
Detection method(s)
Note: The detection methods of the parental variety are shown and expected to be applicable to this variety as well.
Additional Information