CGN-89111-8 - High oleic acid canola | BCH-LMO-SCBD-14780 | Living Modified Organism | Biosafety Clearing-House

Living Modified Organism (LMO)
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last updated: 13 Nov 2012
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High oleic acid canola
23-18-17 (Event 18)
The canola line 23-18-17 was genetically engineered to express modified seed fatty acid content, specifically high levels of lauric acid and myristic acid. The increased levels of lauric acid in oil from the modified canola lines allow for its use as a replacement for other lauric acid oils, such as coconut and palm kernel oil, in products such as confectionery coatings and fillings, margarines, spreads, shortenings and commercial frying oils.

The modified fatty acid content of 23-18-17 is a result of the insertion of a thioesterase (TE) encoding gene from the California bay tree, Umbellularia californica, which is an alternative source of the spice “bay leaf” that is harvested commercially from Lauris nobilis. The introduced thioesterase enzyme is active in the fatty acid biosynthetic pathway of the developing seed and causes the accumulation of triacylglycerides containing esterified lauric acid and, to a lesser extent, myristic acid.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • CGN-89465-2 - High oleic acid canola
    | Monsanto (Calgene) | Production of medical or pharmaceutical compounds (human or animal) (Omega-3 fatty acids (e.g. DHA)), Resistance to antibiotics (Kanamycin)
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-15005-5 Thioesterase | Umbellularia californica (Laurel, California Bay Laurel, UMBCA)
    Protein coding sequence | Production of medical or pharmaceutical compounds (human or animal) (Omega-3 fatty acids (e.g. DHA))
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-104354-1 Napin gene promoter | Brassica rapa (Canola plant)
  • BCH-GENE-SCBD-104355-1 Napin gene terminator | Brassica rapa (Canola plant)
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
  • BCH-GENE-SCBD-104340-2 Tumour Morphology Large gene terminator | Agrobacterium tumefaciens (Agrobacterium)
The original transformation event 23 was estimated to have 15 copies of the genes, at five independent genetic loci, as shown by Southern and segregation analyses. Lines 23-198 and 23-18-17 are several generations removed from the original transformant. Mendelian inheritance, and Southern and PCR analyses of third-generation material show the stability of the introduced genes as the bands did not change position. Some of the copies are segregating out, as expected, so that these lines may not be homogeneous for the number of copies present.
LMO characteristics
Detection method(s)
Additional Information
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