NMK-89761-6 - Atlantic NewLeaf™ potato | BCH-LMO-SCBD-14789 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 02 May 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Atlantic NewLeaf™ potato
EN
ATBT04-6
Yes
NMK-89761-6
The transgenic cultivars of ‘Atlantic’ (ATBT04-6, ATBT04-27, ATBT04-30, ATBT04-31, ATBT04-36) NewLeaf® potatoes were genetically engineered to be resistant to attack by Colorado potato beetle (CPB; Leptinotarsa decemlineata) by producing their own insecticide. These lines were developed by introducing the cry3A gene, isolated from the common  soil bacterium Bacillus thuringiensis subspecies tenebrionis (Btt), into the potato genome.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Cultivar: Atlantic
EN
  • NMK-89279-1 - Atlantic NewLeaf™ potato
    | Monsanto | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Insects, Coleoptera (beetles))
  • NMK-89367-8 - Atlantic NewLeaf™ potato
    | Monsanto | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Insects, Coleoptera (beetles))
  • NMK-89613-2 - Atlantic NewLeaf™ potato
    | Monsanto | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Insects, Coleoptera (beetles))
  • NMK-8917Ø-9 - Atlantic NewLeaf™ potato
    | Monsanto | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Insects, Coleoptera (beetles))
Characteristics of the modification process
PV-STBT04
EN
  • Agrobacterium-mediated DNA transfer
 
1.700 kb
 
 
1.800 kb
 
 
0.630 kb
 
 
0.260 kb
 
 
0.790 kb
 
 
0.320 kb
 
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-14989-5 Cry3A | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Coleoptera (beetles))
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-103851-5 rbcS Promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Promoter
  • BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)
    Terminator
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
The coding sequence of the Cry3A gene was modified to plant preferred codons. This resulted in changes to 399 of 1791 nucleotides but there were no changes to the resulting amino acid sequence.

Southern blot analysis indicated that a three copies of the transformation cassette for the coding sequences of both Cry3A and nptII were transformed into the host genome at 3 different sites. No portions of the vector backbone were detected.
EN
LMO characteristics
EN
  • Food
  • Feed
Detection method(s)
EN
Additional Information
The Cry3A protein expressed in these transgenic potato cultivars is identical to that found in nature and in commercial Bt spray formulations. Cry proteins, of which Cry3A is only one, act by selectively binding to specific sites localized on the lining of the midgut of susceptible insect species. Following binding, pores are formed that disrupt midgut ion flow causing gut paralysis and eventual death due to bacterial sepsis. Cry3A is insecticidal only when eaten by the larvae of coleopteran insects such as Colorado potato beetle and its specificity of action is directly attributable to the presence of specific binding sites in the target insects.
EN
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