FLO-11226-9 - Moonshade™ carnation | BCH-LMO-SCBD-14829 | Living Modified Organism | Biosafety Clearing-House

Loading...
  |  

Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 15 Oct 2015
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Moonshade™ carnation
EN
1226A (11226)
Yes
FLO-11226-9
Carnation has a modified flower colour, a shade of light mauve, whereas the non-GM parent has cream-white flowers. The colour has been achieved by introducing into white carnation two genes of the anthocyanin biosynthesis pathway from Petunia and Viola sp. These genes, encoding dihydroflavonol 4-reductase (dfr) and flavonoid 3'5' hydroxylase (f3'5'h), together with other genes of the anthocyanin biosynthesis pathway already present in the non GM carnation, give rise to the anthocyanins delphinidin and cyanidin.

Note: This line was never commercialised

NOTE: This LMO was formerly referred to with the UID FLO-11226-8.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
  • FLO-11363-2 - Moonshadow™ carnation
    | SUntory Holdings Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Sulfonylurea)
  • FLO-4Ø689-6 - Moonaqua™ carnation
    | Suntory Holdings  Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Sulfonylurea)
  • FLO-4Ø685-2 - Moonvista™ carnation
    | SUntory Holdings Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Sulfonylurea)
  • FLO-11351-8 - Moonshade™ carnation
    | SUntory Holdings Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Sulfonylurea)
  • FLO-114ØØ-3 - Moonshade™ carnation
    | SUntory Holdings Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Sulfonylurea)
  • FLO-11959-4 - Moonshade™ carnation
    | SUntory Holdings Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Sulfonylurea)
  • FLO-11988-6 - Moonshade™ carnation
    | SUntory Holdings Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Sulfonylurea)
Characteristics of the modification process
pCGP1991
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-15177-7 Acetohydroxy acid synthase gene | Nicotiana tabacum (Tobacco, TOBAC )
    Protein coding sequence | Resistance to herbicides (Chlorsulfuron, Sulfonylurea)
  • BCH-GENE-SCBD-15009-4 Dihydroflavonol-4-reductase | Petunia hybrida (Petunia, PETHY)
    Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
  • BCH-GENE-SCBD-43793-4 Flavonoid 3', 5'-hydroxylase gene | Viola sp. (Pansy, VIOLA)
    Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-101901-3 5' untranslated leader of chlorophyll a/b-binding protein | Petunia hybrida (Petunia, PETHY)
    Leader
  • BCH-GENE-SCBD-100390-7 Acetohydroxy acid synthase gene terminator | Nicotiana tabacum (Tobacco, TOBAC )
    Terminator
  • BCH-GENE-SCBD-105798-1 Dihydroflavonol-4-reductase promoter | Petunia hybrida (Petunia, PETHY)
    Promoter
  • BCH-GENE-SCBD-105799-1 Dihydroflavonol-4-reductase terminator | Petunia hybrida (Petunia, PETHY)
    Terminator
  • BCH-GENE-SCBD-103771-1 Chalcone synthase gene promoter | Antirrhinum majus (Common Snapdragon, Snapdragon)
    Promoter
  • BCH-GENE-SCBD-103772-2 D8 gene terminator | Petunia hybrida (Petunia, PETHY)
    Terminator
EN
LMO characteristics
EN
  • Ornamental
Detection method(s)
EN
Additional Information
Transgenic line 1226A was developed using recombinant DNA techniques to produce flowers with a unique deep purple colour by introducing two genes that function together in the biosynthesis of the anthocyanin pigment delphinidin. The transgenic line was derived from the parent cultivar ‘White Unesco’, which is a white coloured carnation that was selected for a mutation in the dihydroflavonol reductase (DFR) encoding gene that did not allow for expression of a functional enzyme, and thus did not produce the anthocyanin type pigments that give rise to blue and red coloured flowers.

The two genes introduced into the transgenic carnation line included a functional dihydroflavonol reductase encoding gene isolated from petunia (Petunia hybrida) and a gene encoding the enzyme flavonoid 3’, 5’-hydroxylase (F3’, 5’H), a member of the NADPH-Cytochrome P450 reductase family, isolated from Viola. Expression of the F3’, 5’H encoding gene (bp40) allows for the production of blue coloured delphinidin anthocyanin pigments, which are not normally found in carnations.

Tolerance to sulfonyl urea herbicides was produced via the introduction of a chlorsulfuron tolerant version of the acetolactate synthase (ALS) encoding gene from tobacco.
EN
Records referencing this document Show in search
Record type Field Record(s)
Living Modified Organism Related LMO(s) 7