FLO-ØØØ15-3 - Moondust™ carnation | BCH-LMO-SCBD-14837 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 15 Oct 2015
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Moondust™ carnation
EN
15
Yes
FLO-ØØØ15-3
Colour-modified carnation produced through introduction of two anthocyanin biosynthetic genes to result in a violet/mauve colouration, the dfr gene which encodes dihydroflavonol reductase and the hf1 gene which encodes flavonoid 3',5'hydroxylase (F3'5'H) from Petunia hybrida.  A variant form of acetolactate synthase (ALS) from Nicotiana tabacum was used as a selectable marker to confer tolerance to sulfonylurea herbicide.

Note: This line was never commercialised

NOTE: This LMO was formerly referred to with the UID FLO-ØØØ15-2.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
  • FLO-Ø7442-5 - Moondust™ carnation
    | SUntory Holdings Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Sulfonylurea)
  • FLO-4Ø619-8 - Moonshade™ carnation
    | SUntory Holdings Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Sulfonylurea), Selectable marker genes and reporter genes
  • FLO-4Ø644-6 - Moonlite™ carnation
    | SUntory Holdings Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Sulfonylurea)
  • FLO-ØØØ16-4 - Moondust™ carnation
    | SUntory Holdings Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Sulfonylurea)
  • FLO-ØØØØ4-1 - Moondust™ carnation
    | SUntory Holdings Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Sulfonylurea)
Characteristics of the modification process
pCGP1470
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-15009-4 Dihydroflavonol-4-reductase | Petunia hybrida (Petunia, PETHY)
    Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
  • BCH-GENE-SCBD-15010-3 Flavonoid 3', 5' hydroxylase gene | Petunia hybrida (Petunia, PETHY)
    Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
  • BCH-GENE-SCBD-15177-7 Acetohydroxy acid synthase gene | Nicotiana tabacum (Tobacco, TOBAC )
    Protein coding sequence | Resistance to herbicides (Chlorsulfuron, Sulfonylurea)
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-100390-7 Acetohydroxy acid synthase gene terminator | Nicotiana tabacum (Tobacco, TOBAC )
    Terminator
  • BCH-GENE-SCBD-103771-1 Chalcone synthase gene promoter | Antirrhinum majus (Common Snapdragon, Snapdragon)
    Promoter
  • BCH-GENE-SCBD-103772-2 D8 gene terminator | Petunia hybrida (Petunia, PETHY)
    Terminator
  • BCH-GENE-SCBD-103773-1 mac-1 Promoter
    Promoter
  • BCH-GENE-SCBD-103774-1 Mannopine synthase gene terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
EN
LMO characteristics
EN
  • Ornamental
Detection method(s)
EN
Additional Information
These carnations were developed using recombinant DNA techniques to produce flowers with a unique violet/mauve colour by introducing two genes from petunia (Petunia hybrida) that function together in the biosynthesis of the anthocyanin pigment delphinidin. The transgenic lines were derived from the parent cultivar ‘White Unesco’, which is a white coloured carnation that was selected for a mutation in the dihydroflavonol reductase (DFR) encoding gene that did not allow for expression of a functional enzyme, and thus did not produce the anthocyanin type pigments that give rise to blue and red coloured flowers. The two genes from Petunia hybrida introduced into the transgenic carnation lines included a functional dihydroflavonol reductase encoding gene (dfr) and a gene (hf1) encoding the enzyme flavonoid 3’, 5’-hydroxylase (F3’5’H), a member of the NADPH-Cytochrome P450 reductase family. Expression of the F3’5’H encoding gene allows for the production of blue coloured delphinidin anthocyanin pigments, which are not normally found in carnations.

Tolerance to sulfonyl urea herbicides was produced via the introduction of a chlorsulfuron tolerant version of the acetolactate synthase (ALS) encoding gene from tobacco (SuRB).
EN
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Record type Field Record(s)
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