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Living Modified Organism (LMO)
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Roundup Ready™ Bollgard II™ cotton
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15985 x 1445 (MON1445 x MON15985)
Yes
MON-15985-7 × MON-Ø1445-2
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Organization:Monsanto ()Phone:Fax:Email:Website:
Insect resistant and herbicide tolerant cotton with stacked events derived by crossing MON-15985-7 and MON-Ø1445-2, including the cry1Ac gene and the cry2Ab gene from Bacillus thuringiensis subsp. kurstaki conferring resistance to lepidopteran pests, and the epsps gene encoding 5-enolpyruvylshikimaete-3-phosphate synthase that conferred tolerance to the herbicide glyphosate.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12080-6 Organism Gossypium hirsutum (Cotton)Crops
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BCH-LMO-SCBD-14774-18 Living Modified Organism MON-15985-7 - Bollgard II™ cotton This document has been updated. This is not the latest published version. Click here to view the latest version of the record.Monsanto | Resistance to antibiotics (Streptomycin), Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths)), Selectable marker genes and reporter genes
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BCH-LMO-SCBD-14880-14 Living Modified Organism MON-Ø1445-2 - Roundup Ready™ cottonMonsanto | Resistance to antibiotics (Kanamycin, Streptomycin), Resistance to herbicides (Glyphosate)
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PV-GHGT07, PV-GHBK11 and PV-GHBK04
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- Cross breeding
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-14986-6 Cry1Ac | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)Protein coding sequence | Resistance to herbicides (Glyphosate)
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BCH-GENE-SCBD-14988-7 Cry2Ab2 | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-103901-2 HSP 70 5' untranslated leader sequence | Petunia hybrida (Petunia, PETHY)Leader
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loading record details...BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Transit signal
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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loading record details...BCH-GENE-SCBD-103856-6 α' subunit of β-conglycinin gene terminator | Glycine max (Soybean, Soya bean, Soya, SOYBN)Terminator
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loading record details...BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-46004-7 Beta-glucuronidase coding sequence | Escherichia coli (ECOLX)Protein coding sequence | Selectable marker genes and reporter genes
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BCH-GENE-SCBD-101507-5 FMV 34S promoter | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)Promoter
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loading record details...BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)Terminator
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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loading record details...BCH-GENE-SCBD-15033-8 3"(9)-O-aminoglycoside adenyltransferase | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Streptomycin)
DNA insert from MON-15985-7 vectors PV-GHBK11 and PV-GHBK04
Event 15985 (tradename Bollgard II®) was derived from the retransformation of transgenic cotton line MON 531. As a result of these two transformation events, MON15985 contains the cry1Ac gene and the cry2Ab conferring resistance to lepidopteran pests as well as copies of the nptII and uidA and aadA genes.
DNA insert from MON-Ø1445-2 vector PV-GHGT07
The MON1445 parental organism contains coding sequences and regulatory elements for epsps and nptII. The aadA gene was also integrated into the host genome, however it is only expressed in bacterial cells. The PV-GHGT07 vector also contained a coding sequence for the Glyphosate oxidoreductase (GOX) gene however southern blot analysis indicated that that it was not integrated into the host genome.
For additional information on this LMO, please refer to the records of the parental LMOs.
EN
Event 15985 (tradename Bollgard II®) was derived from the retransformation of transgenic cotton line MON 531. As a result of these two transformation events, MON15985 contains the cry1Ac gene and the cry2Ab conferring resistance to lepidopteran pests as well as copies of the nptII and uidA and aadA genes.
DNA insert from MON-Ø1445-2 vector PV-GHGT07
The MON1445 parental organism contains coding sequences and regulatory elements for epsps and nptII. The aadA gene was also integrated into the host genome, however it is only expressed in bacterial cells. The PV-GHGT07 vector also contained a coding sequence for the Glyphosate oxidoreductase (GOX) gene however southern blot analysis indicated that that it was not integrated into the host genome.
For additional information on this LMO, please refer to the records of the parental LMOs.
EN
- Food
- Feed
- Fiber/textile
- MON-15985-7 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) [ English ]
- MON-Ø1445-2 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) [ English ]
- EU-RL GMFF validation report [ English ]
- MON-Ø1445-2 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- MON-15985-7 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- MON-15985-7 - CropLife International Detection Methods Database ( CropLife ) [ English ]
- MON-Ø1445-2 - CropLife International Detection Methods Database ( CropLife ) [ English ]
EN
Insect resistant and herbicide tolerant cotton with stacked events derived by crossing MON-15985-7 and MON-Ø1445-2. Insect-resistance was derived from parent 15985 (tradename Bollgard II®) which contained the cry1Ac and cry2Ab genes originally isolated from Bacillus thuringiensis subsp. kurstaki. As a result, event 15985 expresses both the Cry1Ac and Cry2Ab insecticidal proteins. This protects cotton from feeding by a range of Lepidopteran species including tobacco budworm (Heliothis virescens), pink bollworm (Pectinophora gossypiella), cotton bollworm (Helicoverpa zea), cabbage looper (Trichoplusia ni), saltmarsh caterpillar (Estigmene acrea), cotton leaf perforator (Bucculatrix thurbeiella), soybean looper (Pseudoplusia includens), beet armyworm (Spodoptera exigua), fall armyworm (Spodoptera frugiperda), yellowstriped armyworm (Spodoptera ornithogolli) and European corn borer (Ostrinia nubilalis).
As with other B. thuringiensis-derived delta-endotoxins, the Cry1Ac and Cry2Ab proteins exert their insecticidal activity by binding to specific receptors located on the brush border midgut epithelium of susceptible insect species. Following binding, cation-specific pores are formed that disrupt midgut ionic equilibrium leading to gut paralysis and eventual death due to bacterial sepsis. Cry1Ac and Cry2Ab are highly selective and are only active against Lepidopteran insects. These proteins do, however, interact with different receptor sites in the target insects and it is expected that “stacking” these traits will result in increased protection against insect attack and a delay in the development of resistant insect populations.
Herbicide tolerance was derived from parental line 1445 which was transformed to express resistance to glyphosate, the active ingredient in the herbicide Roundup®, allowing for its use as a weed control option. In order to obtain field tolerance to glyphosate herbicide, a bacterial gene encoding a glyphosate-tolerant form of the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) was introduced into the cotton genome.
EN
As with other B. thuringiensis-derived delta-endotoxins, the Cry1Ac and Cry2Ab proteins exert their insecticidal activity by binding to specific receptors located on the brush border midgut epithelium of susceptible insect species. Following binding, cation-specific pores are formed that disrupt midgut ionic equilibrium leading to gut paralysis and eventual death due to bacterial sepsis. Cry1Ac and Cry2Ab are highly selective and are only active against Lepidopteran insects. These proteins do, however, interact with different receptor sites in the target insects and it is expected that “stacking” these traits will result in increased protection against insect attack and a delay in the development of resistant insect populations.
Herbicide tolerance was derived from parental line 1445 which was transformed to express resistance to glyphosate, the active ingredient in the herbicide Roundup®, allowing for its use as a weed control option. In order to obtain field tolerance to glyphosate herbicide, a bacterial gene encoding a glyphosate-tolerant form of the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) was introduced into the cotton genome.
- MON 15985 x MON 1445 - GMO Compass [ English ]
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