NMK-89935-9 - Shepody NewLeaf™ Y potato | BCH-LMO-SCBD-14911 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 29 Apr 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Shepody NewLeaf™ Y potato
Potatoes with insect-resistance and resistance to potato virus Y through inclusion of the cry3A gene from Bacillus thuringiensis which confers resistance to coleopteran pests, and DNA sequences corresponding to potato virus Y (PVY) coat protein domains which confers resistance to PVY.  The nptII gene confers tolerance to the antibiotic kanamycin. 
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Cultivar: Shepody
  • NMK-89653-6 - New Leaf™ Y Russet Burbank potato
    | Monsanto | Resistance to antibiotics (Kanamycin, Streptomycin), Resistance to diseases and pests (Insects, Coleoptera (beetles), Viruses, Potato virus Y (PVY))
  • NMK-8993Ø-4 - Shepody NewLeaf™ Y potato
    | Monsanto | Resistance to antibiotics (Kanamycin, Streptomycin), Resistance to diseases and pests (Insects, Coleoptera (beetles), Viruses, Potato virus Y (PVY))
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-15020-3 PVY coat protein | Potato virus Y (PVY)
    Protein coding sequence | Resistance to diseases and pests (Viruses, Potato virus Y (PVY))
  • BCH-GENE-SCBD-14989-5 Cry3A | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Coleoptera (beetles))
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-15033-8 3"(9)-O-aminoglycoside adenyltransferase | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Streptomycin)
  • BCH-GENE-SCBD-103851-5 rbcS Promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
  • BCH-GENE-SCBD-101507-5 FMV 34S promoter | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)
  • BCH-GENE-SCBD-103922-2 HSP17.9 Leader Sequence | Glycine max (Soybean, Soya bean, Soya, SOYBN)
  • BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)
Integration of the T-DNA occurred at four to five loci. At least one locus contains two copies of the T-DNA organised in inverted orientations and one locus contains two T-DNAs linked by a complete copy of the plasmid backbone. For seven copies of the T-DNA, transfer of the T-DNA resulted in incomplete resolution of the right border leaving incomplete copies of the FMV promoter associated with the PVYcp coding region.

One of the T-DNAs in this line has an incomplete NOS promoter region associated with an intact nptII coding region. One of the nptII genes has a truncation within the coding region. All full length and less than full-length copies of the nptII gene are associated with NOS terminators. The coding regions of all other genetic elements are intact. Plasmid sequences beyond the left and right borders, which include the aad gene and the oriV and ori322 plasmid elements, were inserted into this line. Integration of complete backbone elements occurred in two different ways: at one locus two T-DNAs are linked by a complete copy of the backbone; at two other loci, backbone integration is not associated with the left border flanking the NOS promoter of the nptII gene
LMO characteristics
  • Food
Detection method(s)
Additional Information
The transgenic potato lines SEMT15-02 and SEMT15-15 were produced using recombinant DNA techniques and contain two novel genes, whose individual expression results in resistance to attack by Colorado potato beetle (CPB; Leptinotarsa decemlineata) and resistance to infection by Potato Virus Y strain O (PVY-O). Resistance to attack by CPB was accomplished by introducing the cry3A gene from Bacillus thuringiensis subsp. tenebrionis, which encodes an insecticidal crystalline Cry3A delta-endotoxin protein. The insecticidal activity of Cry3A protein is due to its selective binding to specific sites localized on the brush border midgut epithelium of susceptible insect species. Following binding, cation-specific pores are formed that disrupt midgut ion flow and thereby cause gut paralysis, ultimately leading to bacterial sepsis and death. Delta-endotoxins, such as the Cry3A protein expressed in CPB resistant potato lines, exhibit highly selective insecticidal activity against a narrow range of coleopteran insects such as CPB, elm leaf beetle and yellow mealworm. Their specificity of action is directly attributable to the presence of specific receptors in the target insects. There are no receptors for delta-endotoxins of B. thuringiensis on the surface of mammalian intestinal cells, therefore, livestock animals and humans are not susceptible to these proteins.

Pathogen-derived resistance to PVY was conferred by introducing the coat protein (CP) gene from PVY-O. The coat protein forms a protective coat around the RNA genome of the virus and comprises 95% by mass of the virus particle. Although the exact mechanism is not fully understood, these transgenic potato lines exhibit resistance to infection and subsequent disease caused by PVY through a process that is related to viral cross-protection.