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Living Modified Organism (LMO)
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Enogen™ Maize
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3272
Yes
SYN-E3272-5
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Organization:Syngenta ()Phone:Fax:Email:Website: http://www.syngentaseeds.com,
Maize containing thermostable alpha-amylase (for optimised bioethanol production) through introduction of the amy797E gene from Thermococcales (thermostable bacterium). The pmi gene expresses the PMI protein, which allows the transformed plants to use mannose as an energy source and is used as a selectable marker.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)Crops
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pNOV7013
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- Agrobacterium-mediated DNA transfer
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0.677 kb
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1.383 kb
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0.006 kb
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0.108 kb
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0.070 kb
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1.993 kb
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0.000 kb
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1.176 kb
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0.253 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-14966-7 amy797E alpha amylase | Thermococcales spp. (Thermococcus)Protein coding sequence | Thermostable alpha-amylase,Use in industrial applications (Biofuel production)
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BCH-GENE-SCBD-15003-7 Phosphomannose Isomerase gene | Escherichia coli (ECOLX)Protein coding sequence | Mannose tolerance,Selectable marker genes and reporter genes
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BCH-GENE-SCBD-103622-5 27kD gamma-zein Promoter | Zea mays (Maize, Corn, MAIZE)Promoter
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
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BCH-GENE-SCBD-103627-5 Ubiquitin Intron 1 | Zea mays (Maize, Corn, MAIZE)Intron
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-101406-4 Phosphoenolpyruvate carboxylase, intron 9 | Zea mays (Maize, Corn, MAIZE)Intron
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BCH-GENE-SCBD-102033-4 SEKDEL ER retention signalTransit signal
Information on inserted DNA sequences
Corn Event 3272 contains single copies of the amy797E and pmi genes and single copies of the introduced GZein and ZmUbilnt promoters.
The chimeric amy797E gene was generated to combine the best features of three thermostable amylase enzymes and the coding region of the amy797E gene was modified for the preferred codon usage for maize.
The amy797E gene is fused to a 19 amino acid N-Terminal ER targeting sequence within the promoter sequence of GZein and the SEKDEL C-terminal ER retention signal.
Southern blot analysis indicated that single copies of each of the genetic elements was integrated into the host genome. Analysis indicated that there was no apparent integration of portions of the phosphate backbone.
EN
Corn Event 3272 contains single copies of the amy797E and pmi genes and single copies of the introduced GZein and ZmUbilnt promoters.
The chimeric amy797E gene was generated to combine the best features of three thermostable amylase enzymes and the coding region of the amy797E gene was modified for the preferred codon usage for maize.
The amy797E gene is fused to a 19 amino acid N-Terminal ER targeting sequence within the promoter sequence of GZein and the SEKDEL C-terminal ER retention signal.
Southern blot analysis indicated that single copies of each of the genetic elements was integrated into the host genome. Analysis indicated that there was no apparent integration of portions of the phosphate backbone.
EN
- Biofuel
- SYN-E3272-5 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) [ English ]
- SYN-E3272-5 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- SYN-E3272-5 - CropLife International Detection Methods Database ( CropLife ) [ English ]
EN
EN
- SYN-E3272-5 - Syngenta.pdf [ English ]
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