SYN-E3272-5 - Enogen™ Maize | BCH-LMO-SCBD-15109 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 15 Jun 2017
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Enogen™ Maize
EN
3272
Yes
SYN-E3272-5
Maize containing thermostable alpha-amylase (for optimised bioethanol production) through introduction of the amy797E gene from Thermococcales (thermostable bacterium). The pmi gene expresses the PMI protein, which allows the transformed plants to use mannose as an energy source and is used as a selectable marker.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
Characteristics of the modification process
pNOV7013
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Information on inserted DNA sequences
Corn Event 3272 contains single copies of the amy797E and pmi genes and single copies of the introduced GZein and ZmUbilnt promoters.

The chimeric amy797E gene was generated to combine the best features of three thermostable amylase enzymes and the coding region of the amy797E gene was modified for the preferred codon usage for maize.

The amy797E gene is fused to a 19 amino acid N-Terminal ER targeting sequence within the promoter sequence of GZein and the SEKDEL C-terminal ER retention signal.

Southern blot analysis indicated that single copies of each of the genetic elements was integrated into the host genome. Analysis indicated that there was no apparent integration of portions of the phosphate backbone. 
EN
LMO characteristics
EN
  • Biofuel
Additional Information
EN
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