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Living Modified Organism (LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Herculex™ RW Rootworm Protection maize
EN
59122
DAS-59122-7
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OrganizationDow AgroSciences ()Phone:Fax:Email:
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OrganizationPioneer Hi-Bred International Inc. ()Private sector (business and industry)7100 NW 62nd Avenue PO Box 1000Johnston, Iowa
50131, United States of AmericaPhone: +1 515 535-3200,Fax:Email:Website: www.pioneer.com/,
The transgenic maize line DAS-59122-7 was genetically modified to resist Coleopteran insects (e.g. western corn rootworm, northern corn rootworm and Mexican corn rootworm) and to tolerate herbicides containing glufosinate ammonium.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)Crops
PHP17662
EN
- Agrobacterium-mediated DNA transfer
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1.299 kb
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1.152 kb
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0.318 kb
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0.549 kb
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0.552 kb
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0.199 kb
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Some of these genetic elements may be present as fragments or truncated
forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-14994-9 Cry34Ab1 | Bacillus thuringiensis - Bt, Bacillus, BACTUProtein coding sequence | Resistance to diseases and pests (Insects, Coleoptera (beetles))
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BCH-GENE-SCBD-14995-8 Cry35Ab1 | Bacillus thuringiensis - Bt, Bacillus, BACTUProtein coding sequence | Resistance to diseases and pests (Insects, Coleoptera (beetles))
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BCH-GENE-SCBD-15002-4 Phosphinothricin N-acetyltransferase geneProtein coding sequence | Resistance to herbicides (Glufosinate)
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | (Maize, Corn)Promoter
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BCH-GENE-SCBD-100367-4 Proteinase inhibitor II gene terminator | (Potato)Terminator
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BCH-GENE-SCBD-100368-6 Peroxidase gene promoter | (Wheat)Promoter
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BCH-GENE-SCBD-100287-7 CaMV 35S promoterPromoter
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BCH-GENE-SCBD-100290-6 CaMV 35S terminatorTerminator
The cry34Ab1 and cry35Ab1 were cloned from Bacillus thuringiensis strain PS149B1 and the coding sequence of both genes has been adapted to the codon usage in maize as to achieve expression in the maize plant.
Sequence analysis of 59122 done by the European Food Safety Authority indicated that this LMO contains one complete copy of the T-DNA of PHP17662 without internal rearrangements. All three gene cassettes, cry34Ab1, cry35Ab1 and pat, are intact within the transgenic event. The DNA sequences of the genes in 59122 are identical to those in the original plasmid except for two nucleotide differences in the wheat peroxidise promoter. At the 5’ T-DNA end a deletion of 22 bp is observed and at the 3’ T-DNA end a deletion of 25 bp is observed. The absence of vector backbone in maize 59122 was also demonstrated.
Sequence analysis of 59122 done by the European Food Safety Authority indicated that this LMO contains one complete copy of the T-DNA of PHP17662 without internal rearrangements. All three gene cassettes, cry34Ab1, cry35Ab1 and pat, are intact within the transgenic event. The DNA sequences of the genes in 59122 are identical to those in the original plasmid except for two nucleotide differences in the wheat peroxidise promoter. At the 5’ T-DNA end a deletion of 22 bp is observed and at the 3’ T-DNA end a deletion of 25 bp is observed. The absence of vector backbone in maize 59122 was also demonstrated.
- Food
- Feed
- Biofuel
- DAS-59122-7 - CERA GM Database ( English )
- DAS-59122-7_EFSA.pdf ( English )
- DAS-59122-7_DowAgroSciences.pdf ( English )
- DAS-59122-7 - Dow.pdf ( English )
- DAS-59122-7 - OECD ( English )
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