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Living Modified Organism
(LMO)
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Virus-resistant squash
EN
CZW3
Yes
SEM-ØCZW3-2
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Organization:Monsanto ()800 North Lindbergh Blvd.St. Louis, MO
63167, United States of AmericaPhone: + 1 314 694-1000,Fax: +1 314 694-3080,Email:Website: http://www.monsanto.com, -
Organization:Seminis Vegetable Seeds, Inc. ()Phone:Fax:Email:Website: http://www.seminis.com/,
Squash resistant to infection by Cucumber mosaic cucumovirus (CMV), Zucchini yellow mosaic potyvirus (ZYMV) and Watermelon mosaic potyvirus 2 (WMV-2) through incorporation of virus-derived sequences that encode the coat proteins (CPs) from each of these viruses.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12123-4 Organism Cucurbita pepo (Pumpkin, Squashes)Crops
EN
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SEM-ØZW2Ø-7 - Virus-resistant squash| Monsanto | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Viruses, Mosaic virus, Watermelon mosaic virus-2 (WMV2), Zucchini yellow mosaic virus (ZYMV))
CMV73/ZYMV72/WMBN22
EN
- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-15027-3 CMV coat protein | Cucumber mosaic virus (CMV)Protein coding sequence | Resistance to diseases and pests (Viruses, Mosaic virus, Cucumber mosaic virus (CMV))
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BCH-GENE-SCBD-15025-3 ZYMV coat protein | Zucchini yellow mosaic virus (ZYMV)Protein coding sequence | Resistance to diseases and pests (Viruses, Mosaic virus, Zucchini yellow mosaic virus (ZYMV))
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BCH-GENE-SCBD-15024-4 WMV-2 coat protein | Watermelon mosaic virusProtein coding sequence | Resistance to diseases and pests (Viruses, Mosaic virus, Watermelon mosaic virus-2 (WMV2))
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-101416-7 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
All three coat protein genes were fused to the 5' untranslated sequence from CMV to enhance translation of the transgene mRNA.
EN
EN
- Food
Southern blot analysis of genomic DNA from CZW-3 indicated the presence of a single copy of the T-DNA containing intact sequences corresponding to the CP encoding genes form CMV, ZYMV, and WMV2, integrated at a single site. Additional analyses verified the presence of a single copy of the NPTII encoding neo antibiotic resistance marker gene, and no incorporation of plasmid backbone sequences outside of the T-DNA region.
EN
The CZW-3 squash line was developed using recombinant DNA techniques to resist infection by CMV, ZYMV, and WMV2 by inserting virus-derived sequences that encode the coat proteins (CPs) from each of these viruses. The introduced viral sequences do not result in the formation of any infectious particles, nor does their expression result in any disease pathology.
This transgenic squash exhibits “pathogen-derived resistance” to infection and subsequent disease caused by CMV, ZYMV, and WMV2 through a process that is related to viral cross-protection. Although the exact mechanism by which the viral protection occurs is unknown, most evidence suggests that expression of viral CP by a plant interferes with one of the first steps in viral replication, uncoating (removal of CP) from the incoming virus. Other modes of action of cross-protection have also been suggested.
EN
This transgenic squash exhibits “pathogen-derived resistance” to infection and subsequent disease caused by CMV, ZYMV, and WMV2 through a process that is related to viral cross-protection. Although the exact mechanism by which the viral protection occurs is unknown, most evidence suggests that expression of viral CP by a plant interferes with one of the first steps in viral replication, uncoating (removal of CP) from the incoming virus. Other modes of action of cross-protection have also been suggested.
- SEM-ØCZW3-2 - OECD [ English ]
- CZW-3 - APHIS.pdf [ English ]
| Record type | Field | Record(s) | |
|---|---|---|---|
| Country's Decision or any other Communication | LMO identification | 1 | |
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 1 | |
| Living Modified Organism | Related LMO(s) | 1 | |
