SY-GTSB77-7 - InVigor™ Sugar Beet | BCH-LMO-SCBD-15409 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 13 Jul 2006 last updated: 27 Aug 2015
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
InVigor™ Sugar Beet
EN
GTSB77
Yes
SY-GTSB77-7
Sugar beet line 77 was produced to exhibit tolerance to the herbicide glyphosate (Roundup) by incorporating the 5-enolpyruvylshikimate-3-phosphate synthase (cp4 epsps) gene from Agrobacterium sp. strain CP4 and a glyphosate oxidoreductase gene (gox) from Ochrobactrum anthropi.

NOTE: This LMO was formerly referred to with the UID SY-GTSB77-8.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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Characteristics of the modification process
PV-BVGT03
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)
    Protein coding sequence | Resistance to herbicides (Glyphosate)
  • BCH-GENE-SCBD-14998-4 Glyphosate oxidoreductase gene | Ochrobactrum anthropi (OCHAN)
    Protein coding sequence | Resistance to herbicides (Glyphosate)
  • BCH-GENE-SCBD-46004-7 Beta-glucuronidase coding sequence | Escherichia coli (ECOLX)
    Protein coding sequence | Selectable marker genes and reporter genes
  • BCH-GENE-SCBD-101416-6 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
  • BCH-GENE-SCBD-101507-5 FMV 34S promoter | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)
    Promoter
  • BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Transit signal
  • BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)
    Terminator
  • BCH-GENE-SCBD-101902-4 rbcS Transit Peptide | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Transit signal
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
  • BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
Information on the inserted DNA sequences:
The codon optimised cp4epsps coding sequence cassette consists of the figwort mosaic virus (FMV) promoter, a chloroplast targeting sequence from Arabidosis thaliana, a CP4 EPSPS coding region from Agrobacterium sp. strain CP4. The CP4 EPSPS protein is highly resistant to inhibition by glyphosate, the active ingredient in Roundup herbicide.The uidA coding region for the B-D-glucuronidase (GUS) protein from E. coli and the 3’ non-translated region from pea which directs polyadenylation. This gene serves as a selectable marker gene during the plant transformation process.The gox coding sequence cassette consists of the figworth mosaic virus (FMV) promoter, a chloroplast targeting sequence from Arabidosis thaliana promoter coding region from Ochrobactrum anthropi and the 3’ non-translated region of  the nopaline synthase gene, which directs polyadenylation. When expressed, the function of the glyphosate oxidase (GOX) enzyme is to metabolize glyphosate (N-phosphonomethylglycine), the active ingredient in Roundup herbicide to an inactive form.

Vector information
The plasmid vector PV-BVGT03 contains well characterized DNA segments required for selection and replication of the plasmid in the bacteria as well as a right border for initiating the region of T-DNA, into the plant genomic DNA. The plasmids are composed of several genetic elements: cp4 epsps coding sequence from Agrobacterium tumefaciens, uid sequence (GUS) from Escherichi coli, gox sequence from Ochrobactrum anthropi, and nptII coding sequence from Tn5. The nptII gene was not transferred into the sugar beet genome because of the truncation of the insertion event within the gox gene. In addition, the plasmid contains a bacterial selectable marker coding sequence, aad as well as origins of replication (ori-V and ori-322) necessary for replication and maintenance of the plasmid PV-BVGT03 in bacteria.

The truncated nptII construct was as follows: CaMV 35S promoter >> nptII gene >> nos terminator
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LMO characteristics
EN
  • Food
  • Feed
Detection method(s)
Southern blot analysis indicates that a single copy of the epsps gene integrated into the host genome in addition to single copies of the gus and gox expression cassettes. However nuleotide sequencing analyses indicated that the gox coding sequence did not fully integrate into the host genome and is thus present in a truncated form. Protein expression analysis further indicated that the gox is expressed in a truncated, chimeric and incative form.
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Additional Information
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