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Living Modified Organism (LMO)
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Tobacco modified for reduced nicotine levels
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Vector 21-41
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Organization:Vector Tobacco Ltd ()Phone:Fax:Email:Website: http://www.vectortobacco.com/,
Tobacco which expresses a low level of nicotine through incorporation of an antisense version of the Quinolinic Acid Phosphoribosyltransferase gene (NtQPT1) which codes for for quinolinic acid phosphoribosyltransferase (QPTase). The nptII gene confers resistance to the antibiotic kanamycin and was used as a marker gene.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12120-4 Organism Nicotiana tabacum (Tobacco, TOBAC )Crops
Tobacco variety Burley 21-LA
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pYTY32 derived from pBin19
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-15416-2 Quinolinic Acid Phosphoribosyltransferase gene | Nicotiana tabacum (Tobacco, TOBAC )Protein coding sequence | Reduced nicotine content
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-101416-6 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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loading record details...BCH-GENE-SCBD-104448-1 Quinolinic Acid Phosphoribosyltransferase gene Promoter | Nicotiana tabacum (Tobacco, TOBAC )Promoter
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BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
Vector 21-41 tobacco has been genetically engineered to express a quinolinic acid phosphoribosyltransferase (QPTase) in the reverse, or antisense position, which disrupts the normal expression of QPTase, a key enzyme in the biosynthetic pathway leading to the production of nicotine and related alkaloids. The effect of this genetic change is to reduce the nicotine levels of nicotine, nor-nicotine, and total alkaloids in the leaves of Vector 21-41 tobacco.
The tobacco line also contains the nptII marker gene derived from the bacterium Escherichia coli. The nptII gene encodes the enzyme neomycin phosphotransferase type II (NPTII) and is used as a selectable marker in the initial laboratory stages of plant cell selection. Expression of the added genes is controlled in part by gene sequences from the plant pathogen Agrobacterium tumefaciens. The A. tumefaciens method was used to transfer the added genes into the parental recipient tobacco variety.
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The tobacco line also contains the nptII marker gene derived from the bacterium Escherichia coli. The nptII gene encodes the enzyme neomycin phosphotransferase type II (NPTII) and is used as a selectable marker in the initial laboratory stages of plant cell selection. Expression of the added genes is controlled in part by gene sequences from the plant pathogen Agrobacterium tumefaciens. The A. tumefaciens method was used to transfer the added genes into the parental recipient tobacco variety.
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- US EPA Federal Register [ English ]
- Vector 21 41.pdf [ English ]
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