Tomato modified for delayed ripening | BCH-LMO-SCBD-15419 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 13 Jul 2006 last updated: 08 Apr 2014
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Tomato modified for delayed ripening
Tomato with delayed ripening due to expression of the SAMase from the E. coli bacteriophage T3.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-15017-5 S-adenosylmethionine hydrolase gene | Bacteriophage T3 (Phage T3, T3)
    Protein coding sequence | Changes in physiology and/or production (Ripening)
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
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    BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
  • BCH-GENE-SCBD-104862-2 E8 gene promoter | Solanum lycopersicum (Tomato, SOLLC)
The sam-k gene was modified to alter the native initiation site to contain a consensus eukaryotic translation initiation site - Kozak sequence.

Southern blot ananlysis indicated that the T-DNA integrated at a single locus within the host genome. Within this site is one complet and on e partial copy of the sam-k coding sequence. Further more fragments of the oriT and TrfA genetic elements from the vector backbone were detected in the host genome.
LMO characteristics
  • Food
Detection method(s)
Additional Information
The large red cherry tomato Lycopersicon esculentum mill var. cerasiforme has a modified ripening phenotype resulting in the production of a more physiologically mature fruit that is able to withstand the rigors of the current production and distribution system. This was accomplished by inserting the sam-k gene encoding SAMase from the E. coli bacteriophage T3 (under a promoter that directs fruit-specific expression) into the tomato genome. SAMase degrades S-adenosylmethionine (SAM), which is the penultimate precursor in the ethylene biosynthetic pathway.

The nptII enzyme was used as a selectable marker in the production of the transgenic tomatoes.