Loading...
This record has been deleted. Since it is linked to a published record, it is available here for reference purposes only.
Click here to view the deleted recordThis document has been updated. This is not the latest published version. Click here to view the latest version of the record.
Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Insect resistant, herbicide tolerant maize
EN
DBN9936
No
-
Organization:Beijing Dabeinong Biotechnology Co., Ltd. (DBNBC)No. 2, Old Summer Palace West RoadBeijing, Haidan
100193, ChinaPhone: +86 010-82408600,Fax: +86 010-82408644,Email: hr-biotech@dbn.com.cn, cwbgs@dbn.com.cn,Website: http://www.dbnbc.cn/en,
The maize (Zea mays) was modified for insect resistance and herbicide tolerance. For Lepidoptera resistance, the maize expresses the Bacillus thuringiensis crystal protein Cry1Ab, which causes cell lysis of the insect's midgut through pore formation. For glyphosate tolerance, the maize expresses Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase, which does not bind the herbicide with high affinity and allows for the continued synthesis of aromatic amino acids via the shikimate pathway.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
-
BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)Crops
EN
DBN10124
EN
- Agrobacterium-mediated DNA transfer
|
0.077 kb
|
|
0.253 kb
|
|
2.457 kb
|
|
0.806 kb
|
|
0.328 kb
|
|
1.418 kb
|
|
0.228 kb
|
|
1.368 kb
|
|
0.255 kb
|
|
0.152 kb
|
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
-
BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
-
BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
-
BCH-GENE-SCBD-14985-12 Cry1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
-
BCH-GENE-SCBD-100359-7 Hsp70 intron | Zea mays (Maize, Corn, MAIZE)Intron
-
BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
-
BCH-GENE-SCBD-100364-5 Rice actin 1 gene promoter | Oryza sativa (Rice, ORYSA)Promoter
-
BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Transit signal
-
BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)Protein coding sequence | Resistance to herbicides (Glyphosate)
-
BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
-
BCH-GENE-SCBD-101416-7 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
The modified maize contains two gene cassettes: Bacillus thuringiensis crystal (cry1Ab) and Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthases (epsps).
The cry1Ab coding sequence is under control of a Cauliflower mosaic virus (CAMV) 35S promoter and an A. tumefaciens nopaline synthase terminator. A maize heat shock protein 70 intron is also present to enhance the expression of cry1Ab.
The epsps coding sequence is under control of an Oryza sativa actin 1 promoter and a CaMV 35S terminator. An Arabidopsis thaliana chloroplast transit peptide 2 sequence is present for targeting the EPSPS protein to the chloroplasts.
The cry1Ab coding sequence is under control of a Cauliflower mosaic virus (CAMV) 35S promoter and an A. tumefaciens nopaline synthase terminator. A maize heat shock protein 70 intron is also present to enhance the expression of cry1Ab.
The epsps coding sequence is under control of an Oryza sativa actin 1 promoter and a CaMV 35S terminator. An Arabidopsis thaliana chloroplast transit peptide 2 sequence is present for targeting the EPSPS protein to the chloroplasts.
EN
- Feed
- Food
EN
EN
- WO2016173361A1 - Maize plant DBN9936 and method for use in detecting nucleic acid sequence thereof.pdf [ Chinese ]
- EUginius - DBN9936 maize [ English ]
- Expression profiles of Cry1Ab protein and its insecticidal efficacy against the invasive fall armyworm for Chinese domestic GM maize DBN9936.pdf [ English ]
