MON-95379-3 - Insect-protected maize | BCH-LMO-SCBD-258895 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 14 Jan 2022
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Insect-protected maize
The maize (Zea mays) was modified for resistance to Lepidoptera insects and to overcome Bt-resistance in insect pests. The maize expresses synthetic Cry1B.868 and Cry1Da_7 proteins (originally derived from Bacillus thuringiensis), which have a pore-forming mode of action that is independent of the receptors that other Bt toxins interact with. A selectable marker cassette (glyphosate tolerance) was removed using Cre-lox excision during the development of this line to result in a marker-free line. 
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-103069-3 loxP recombination site | Bacteriophage P1 (Phage P1)
    recombination site
  • BCH-GENE-SCBD-258891-1 Lipid transfer protein-like terminator | Oryza sativa (Rice, ORYSA)
  • BCH-GENE-SCBD-258889-1 Cry1B.868 | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), Fall armyworm (Spodoptera frugiperda))
  • BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)
  • BCH-GENE-SCBD-105196-2 FMV 35S Enhancer | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)
  • BCH-GENE-SCBD-258892-1 Tonoplast integral protein 1 promoter | Setaria italica - Foxtail millet, Italian millet
  • BCH-GENE-SCBD-258893-1 Rice actin 15 intron | Oryza sativa (Rice, ORYSA)
  • BCH-GENE-SCBD-258890-1 Cry1Da_7 | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), Cotton bollworm (Helicoverpa spp.), European corn borer (Ostrinia nubilalis), Fall armyworm (Spodoptera frugiperda))
  • BCH-GENE-SCBD-258894-1 GOS2 terminator | Oryza sativa (Rice, ORYSA)
  • BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
The modified maize contains two gene cassettes: synthetic cry1B.868 and synthetic cry1Da_7.

The cry1B.868 coding sequence is under control of a Zea mays ubiquitin promoter and an Oryza sativa lipid transfer-like protein terminator. The promoter contains the promoter, leader and intron sequences from the maize ubiquitin gene. High levels of transcription are expected in all tissues due to the constitutive nature of the promoter.

The cry1Da_7 coding sequence is under control of a Setaria italica promoter and an O. sativa gos2 terminator. The first intron of the rice actin 15 gene was also included and likely improves expression of the gene. 

  • Both cry1B.868 and cry1Da_7 sequences are derived from Bacillus thuringiensis sequences. Refer to the genetic element records for more information.
  • During development of the modified maize, a c4-epsps cassette (rice tubulin A terminator; cp4-epspsArabidopsis thaliana chloroplast transit peptide 2; and rice tubulin A promoter) was removed using Cre-lox excision. The T-DNA right border was also truncated (lost) during transformation.
  • Next-generation sequencing indicated that a single, intact copy of the the intended DNA insertion was present in the parental genome. No backbone or other unexpected sequences were detected.
LMO characteristics
  • Feed
  • Food
Detection method(s)
Records referencing this document Show in search
Record type Field Record(s)
Living Modified Organism Recipient Organism” or “Parental Organisms 1