Insect-resistant cotton | BCH-LMO-SCBD-258904 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 17 Jan 2022
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Insect-resistant cotton
EN
NIBGE‑1601
  • - Organization: National Institute for Biotechnology and Genetic Engineering (NIBGE) | BCH-CON-SCBD-258897-1
    Organization
    National Institute for Biotechnology and Genetic Engineering (NIBGE)
    Academic or research institute
    P.O. Box 577, Jhang Road
    Faisalabad, Punjab
    44000, Pakistan
    Phone: +92 41 920 131 620,
    Fax: +92 041-9201322,
The cotton (Gossypium hirsutum) was modified for resistance to Lepidoptera insects through the expression of Bacillus thuringiensis crystal proteins Cry1Ac and Cry2Ab2. Upon protease cleavage and activation in the insect's midgut, the bioactive core toxins form pores, which disrupt osmotic balance and eventually result in cell lysis and insect death. In addition to the insecticidal proteins, the cotton expresses an Escherichia coli neomycin phosphotransferase II cassette for kanamycin selection during transformation. 
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Coker 312 cultivar
EN
Characteristics of the modification process
pGA482
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
The modified cotton contains three gene cassettes: Escherichia coli neomycin phosphotransferase II (nptII); Bacillus thuringiensis cry2Ab2; and B. thuringiensis cry1Ab.

The nptII coding sequence is under control of an Agrobacterium nopaline synthase promoter and terminator.

The cry2Ab2 coding sequence is under control of a Figwort mosaic virus 34S promoter and an A. tumefaciens transcript 7 gene 3' untranslated region. An Arabidopsis thaliana 5-enolpyruvylshikimate-3-phosphate synthase signal sequence (chloroplast transit peptide 2) was added to target the translated Cry2Ab2 protein to the chloroplast.

The cry1Ac coding sequence is under control of a Cauliflower mosaic virus 35S enhanced promoter and 35S terminator. Due to the duplicated enhancer regions and the constitutive nature of the promoter high levels of expression are expected. 

Note:
  • The nptII cassette was originally sourced from pGA482. The base pair sizes of the genetic elements in this cassette were not found. 
EN
LMO characteristics
EN
  • Fiber/textile
Additional Information
Please note that the GenBank sequence contains an additional cassette (epsps) that is not present in the NIBGE-1601 cotton line.
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