Late blight-resistant Diamant potato | BCH-LMO-SCBD-260844 | Living Modified Organism | Biosafety Clearing-House
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Living Modified Organism (LMO)
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Decisions on the LMO Risk Assessments  
last updated: 28 Jun 2022
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Late blight-resistant Diamant potato
EN
DIA_MSU_UB015
No
The potato (Solanum tuberosum) was modified for late blight resistance. To achieve this, the potato expresses three Phytophthora infestans resistance genes from Solanum venturii (Rpi-vnt1), Solanum mochiquense (Rpi-mcq1) and Solanum bulbocastanum (Rpi-blb2), which recognize host pathogen factors and induce a hypersensitive response. In addition to these resistance genes, the potato expresses Escherichia coli neomycin phosphotransferase II for kanamycin selection during transformation.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Variety Diamant
EN
Characteristics of the modification process
pSIM4392
EN
  • Agrobacterium-mediated DNA transfer
 
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
The modified potato contains four gene cassettes: Escherichia coli neomycin phosphotransferase II (nptII); Solanum venturii Resistance to Phytophthora infestans Resistance gene 1 (Rpi-vnt1); Solanum mochiquense Resistance to Phytophthora infestans resistance gene (Rpi-Mcq1) and Solanum bulbocastanum Resistance to Phytophthora infestans Resistance gene 2 (Rpi-blb2).

The nptII coding sequence is under control of a potato ubiquitin 7 promoter and Rhizobium radiobacter nopaline synthase terminator. The ubiquitin 7 promoter contains the first intron and exon of ubiquitin 7 to enhance the expression of nptII. Between the nptII cassette and the left border region is a Cauliflower mosaic virus 35S terminator, which was sourced from a vector (pCAMBIA1301) used for the construction of pSIM4392.

The Rpi-vnt1, Rpi-Mcq1 and Rpi-blb2 coding sequences are under control of their native promoters and terminators. 

Note:
  • The genetic element sizes are expected as per the transformation vector.
EN
LMO characteristics
EN
  • Food
  • Research
Detection method(s)
EN
Additional Information
For information on the potato variety, kindly refer to the links in "Any other relevant documents" below.
EN
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1

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