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Living Modified Organism (LMO)
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BCH-LMO-SCBD-265213-4   |   PDF   |   Print   |  
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Decisions on the LMO Risk Assessments  
published: 24 Aug 2023 last updated: 16 Jul 2026
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Insect-resistant and herbicide-tolerant maize
EN
DAS1131
Yes
DAS-Ø1131-3
The maize (Zea mays) was modified for resistance to insects and tolerance to herbicides. For resistance to Lepidopteran pests, the modified maize expresses a Bacillus thuringiensis chimeric delta endo-toxin Cry1Da2, which forms non-specific, ion conducting pores in the apical membrane of the midgut epithelial cells and leads to larval death. For tolerance to glyphosate, the modified maize expresses Streptomyces sviceus DGT28 5-enolpiruvilshikimate- 3-phosphate synthase, which allows for the continuing functioning of the shikimate pathway by avoiding inhibition from the herbicidal compound.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Public inbred line B104
EN
Characteristics of the modification process
PHP88492
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)
    Promoter
  • BCH-GENE-SCBD-103927-2 Ubiquitin 5' Untranslated Region | Zea mays (Maize, Corn, MAIZE)
    Leader
  • BCH-GENE-SCBD-103627-5 Ubiquitin Intron 1 | Zea mays (Maize, Corn, MAIZE)
    Intron
  • BCH-GENE-SCBD-110612-2 Ubiquitin gene terminator | Zea mays (Maize, Corn, MAIZE)
    Terminator
  • BCH-GENE-SCBD-265210-2 DGT28 5-enolpiruvilshikimate- 3-phosphate synthase | Streptomyces sviceus (STRXS)
    Protein coding sequence | Resistance to herbicides (Glyphosate)
  • BCH-GENE-SCBD-265211-1 Chimeric chloroplast transit peptide TraP8 | Brassica napus (Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA) | Brassica rapa (Canola plant)
    Transit signal
  • BCH-GENE-SCBD-299352-1 Cry1Da2_1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), Cotton bollworm (Helicoverpa spp.), European corn borer (Ostrinia nubilalis), Fall armyworm (Spodoptera frugiperda))
The modified maize (Zea mays) contains two gene cassettes: Bacillus thuringiensis cry1Da2 and Streptomyces sviceus DGT28 5-enolpiruvilshikimate- 3-phosphate synthase (dgt28-epsps).

The cry1Da2 coding sequence is under control of a Zea mays ubiquitin (ubi1) promoter and terminator. The 5' untranslated region contains a ubi1 leader and intron to enhance the expression of cry1Da2. Due to the nature of the promoter, high levels of expressions are expected from this cassette.

The dgt28-epsps coding sequence is under control of the a ubi1 promoter and terminator. The 5' untranslated region contains a ubi1 leader and intron to enhance the expression of dgt28-epsps. A chimeric transit peptide (TraP8) was added to direct the translated DGT28-EPSPS protein to the chloroplast. Due to the nature of the promoter, high levels of expressions are expected from this cassette.

Note
  • The cry1Da2 coding sequence is a chimeric sequence consisting of cry1Da2 gene encoding an insecticidal core toxin and a derivative of the cry1Ab gene.
  • The TraP8 coding sequence is a chimeric sequence consisting of both Brassica napus and Brassica rapa donor organisms.
  • The Cry1Da2 protein is 603 amino acids in length, including the last 9 amino acids derived from cry1Ab, and has a molecular weight of approximately 68 kDa.
  • The DGT28-EPSPS protein has a length of 481 amino acids and a molecular weight of approximately 51 kDa which includes the 65-amino acid TraP8 peptide as well as a 2-amino acid linker.
  • The T-DNA contains two attB recombination sites, two engineered landing pad regions and four zinc finger nuclease target recognition sites.
  • Southern-by-Sequencing analysis confirmed a single insertion of the T-DNA into the maize genome and the absence of plasmid backbone sequences. No unexpected insertions, deletions, rearrangements or junctions were detected.
  • PCR confirmed presence of the transgenic cassettes, and southern analysis indicated stable inheritance across generations during development.
EN
LMO characteristics
EN
  • Feed
  • Food
Detection method(s)
EN
Additional Information
  • Transformation method: Immature maize embryos were harvested from a surface‐sterilized ear of B104 maize approximately 10‐14 days after pollination and inoculated with Agrobacterium tumefaciens strain DAt13192. Glyphosate was applied to select for transformants. 
  •  DAS-01131-3 seed was deposited with the American Type Culture Collection on May 26, 2021, as ATCC PTA-127077.
EN
Records referencing this document Show in search
Record type Field Record(s)
Living Modified Organism Recipient Organism or Parental Organisms 1
Country's Decision or any other Communication LMO identification 7
Risk Assessment generated by a regulatory process Living modified organism(s) 7

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