DNA insert from 4114 (DP-ØØ4114-3) vector PHP27118
The DNA insert contains four gene cassettes: Bacillus thuringiensis cry1F, B. thuringiensis cry34Ab1, B. thuringiensis cry35Ab1 and Streptomyces viridochromogenes phosphinothricin N-acetyltransferase (pat). 

 

Transcription of the cry1F is under control of a Zea mays ubiquitin promoter and an Agrobacterium tumefaciens ORF25 terminator. A Z. mays ubiquitin intron 1 sequence is also present to enhance the expression of cry1F. Transcription of the cry34Ab1 is under control of a Z. mays ubiquitin promoter and Solanum tuberosum proteinase inhibitor II terminator. A Z. mays ubiquitin intron 1 sequence is also present to enhance the expression of cry34Ab1. Transcription of cry35Ab1 is under control of Triticum aestivum peroxidase promoter and S. tuberosum proteinase inhibitor II terminator. Transcription of pat is under control of Cauliflower mosaic virus (CaMV) 35S promoter and CaMV 35S terminator.
 
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DNA insert from MON87411 (MON-87411-9) vector PV-ZMIR10871
The MON87411 genome contains three cassettes: an RNA interference (RNAi) cassette targeting Diabrotica virgifera virgifera, Bacillus thuringiensis cry3Bb1 and Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (cp4-epsps).

Transcription of the RNAi cassette commences from the Cauliflower mosaic virus 35S enhanced promoter and terminates at the Pisum sativum ribulose bisphosphate carboxylase small chain 2 terminator. The transcript initially contains a Zea mays heat shock protein 70 intron, which contributes to enhanced expression in vegetative tissues of the plant, and two partial coding sequences of the D. virgifera virgifera Snf7p gene, which encodes the SNF7 subunit of the ESCRT-III complex. The two Snf7p sequences are in an inverted orientation, separated by a 150-nucleotide intervening sequence, which allows base pairing between the inverted sequences and hairpin RNA formation post-transcription, which then triggers an RNAi response. Due to RNAi processing, small interfering RNA molecules (roughly 21-23 nucleotides in length) will be produced and thus no translation into protein will occur from this cassette.

Transcription of the cry3Bb1 is under control of the Z. mays physical impedance induced protein promoter and Triticum aestivum (wheat) heat shock protein 17.3 terminator. The transcript also contains a wheat 5' untranslated leader from chlorophyll a/b-binding protein and Oryza sativa actin 1 intron for enhanced expression of the transgene. Expression of cp4-epsps is under control of an O. sativa alpha tubulin promoter and terminator. The transcript additionally contains Arabidopsis thaliana chloroplast targeting peptide 2 to sequester the protein to the chloroplast.
 
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