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Living Modified Organism
(LMO)
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Poplar with decreased lignin content
EN
TRA2A-TRA2B
No
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- Organization: VIB - Flanders Interuniversity Institute for Biotechnology () | BCH-CON-SCBD-42293-1Organization:VIB - Flanders Interuniversity Institute for Biotechnology ()Rijvisschestraat 120 9052 Zwijnaarde, Belgium Tel.: +09 244 66 11,
, BelgiumPhone:Fax: +09 244 66 10,Email: vib@vib.be,Website: www.vib.be,
The modified hybrid poplars (Populus canescens; Populus tremula x Populus alba) have an altered wood composition resulting from the introduction of a knock-out mutation in the endogenous transaldolase genes tra2a and tra2b. These genes are involved in the pentose phosphate pathway that is upstream of the monolignol pathway, which is the basis for the biosynthesis of lignin. The modified trees additionally contain higher levels of cellulose in their cell walls and overall wood composition. The trees also contain a non-functional CRISPR-associated endonuclease from Streptococcus pyogenes and an Escherichia coli hygromycin B phosphotransferase selectable marker.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-101269-5 Organism Populus canescens (Gray poplar, Grey poplar, Hybrid poplar)Trees
Poplar female clone 717-1B4
EN
Cr030
EN
- Agrobacterium-mediated DNA transfer
- Gene editing (e.g. CRISPR-Cas, etc.)
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-277309-1 Synthetic cas9 guide RNAGuide RNA
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BCH-GENE-SCBD-277308-1 Synthetic tra2b guide RNAGuide RNA
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BCH-GENE-SCBD-277307-1 Synthetic tra2a guide RNAGuide RNA
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BCH-GENE-SCBD-277306-1 CRISPR-associated endonuclease 9 | Streptococcus pyogenes (STRPY)Protein coding sequence | Genome editing
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BCH-GENE-SCBD-277304-1 Transaldolase 2a | Populus canescens (Gray poplar, Grey poplar, Hybrid poplar)Protein coding sequence | Changes in quality and/or metabolite content (Cellulose, Lignin)
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BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)Terminator
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BCH-GENE-SCBD-14991-8 Hygromycin B phosphotransferase gene | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Hygromycin),Selectable marker genes and reporter genes
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-277305-1 Transaldolase 2b | Populus canescens (Gray poplar, Grey poplar, Hybrid poplar)Protein coding sequence | Changes in quality and/or metabolite content (Cellulose, Lignin)
The modified poplars contain cassettes for Streptococcus pyogenes CRISPR-associated endonuclease 9 (cas9), synthetic guide RNAs and Escherichia coli hygromycin B phosphotransferase (hph).
The cas9 coding sequence is under control of a Cauliflower mosaic virus 35S promoter and a Pisum sativum ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit terminator. The cas9 sequence contains a mutation to make the final protein product non-functional. During the transformation of the poplars, the CAS9 protein was initially active. However, with the presence of the guide RNA targeting its own sequence, a mutation was induced to produce a final non-functional protein product.
The hph coding sequence is under control of the Agrobacterium tumefaciens nopaline synthase promoter and terminator.
Note:
The cas9 coding sequence is under control of a Cauliflower mosaic virus 35S promoter and a Pisum sativum ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit terminator. The cas9 sequence contains a mutation to make the final protein product non-functional. During the transformation of the poplars, the CAS9 protein was initially active. However, with the presence of the guide RNA targeting its own sequence, a mutation was induced to produce a final non-functional protein product.
The hph coding sequence is under control of the Agrobacterium tumefaciens nopaline synthase promoter and terminator.
Note:
- Information was not provided about the promoters for the guide RNA sequences.
- No vector backbone sequences were integrated into the poplar genome.
After transformation, the functional CAS9 protein complexes with the guide RNAs to induce mutations in the endogenous transaldolase 2a and transaldolase 2b, as well as the cas9 sequence itself. The genome editing results in gene products that are not functional.
- Research
- Other (Pulp and paper)
EN
EN
| Record type | Field | Record(s) | |
|---|---|---|---|
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 1 | |
