MON-89788-1 - Roundup Ready2Yield™ soybean | BCH-LMO-SCBD-40284 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 10 Aug 2007 last updated: 08 Feb 2019
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Roundup Ready2Yield™ soybean
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MON89788
Yes
MON-89788-1
MON89788 is a second-generation glyphosate-tolerant soybean product, was developed by introduction of the cp4 epsps gene cassette consisting of chimeric promoter sequence derived from Arabidopsis thaliana Tsf1 gene promoter and enhancer sequences and from the 35S of the Figwort Mosaic Virus. In addition, the transformation was based on a new technique of Agrobacterium-mediated gene delivery to soybean meristem, where cells were induced directly to form shoots and give rise to transgenic plants
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Variety: A3244
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Characteristics of the modification process
PV-GMGOX20
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  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)
    Protein coding sequence | Resistance to herbicides (Glyphosate)
  • BCH-GENE-SCBD-103903-1 Elongation factor EF-1alpha promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Promoter
  • BCH-GENE-SCBD-103904-1 Elongation factor EF-1alpha Leader | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Leader
  • BCH-GENE-SCBD-103905-1 Elongation factor EF-1alpha Intron 1 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Intron
  • BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Transit signal
  • BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)
    Terminator
  • BCH-GENE-SCBD-105196-2 FMV 35S Enhancer | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)
    Leader
Information on the inserted DNA sequences:
The DNA inserted into the soybean genome contains:
- Codon optimized coding sequence of the aroA (epsps) gene from the Agrobacterium sp. strain CP4 encoding the CP4 EPSPS protein.
- a chimeric transcriptional promoter (P-FMV/Tsf1) consisting of chimeric sequence derived from Arabidopsis thaliana Tsf1 gene promoter and enhancer sequences from  the 35S of the Figwort Mosaic Virus. Located between the promoter and the cp4epsps coding sequence are the non-translated leader sequence (L-Tsf1) and the I-Tsf1 non translated intron;
- a chloroplast transit peptide sequence (TS-CTP2), derived from the Arabidopsis thaliana epsps gene and placed before the cp4 epsps gene encoding sequence in the gene insert;
- a polyadenylation sequence from RbcS2 gene (T-E9), derived from Pisum sativum containing the 3’ non translated region of the pea ribulose-1,5 biphosphate carboxylase small sub unit E9.

Vector information
The PV-GMGOX20 vector is approximately 9.7 kb and contains a cp4 epsps gene expression cassette delineated by left and right border regions. The T-DNA (transferred DNA) that is incorporated into the soybean genome is approximately 4.3 kb, and the DNA backbone region that is not incorporated into the soybean genome is approximately 5.4kb.

Southern blot analyses indicate that MON 89788 contains a single intact cp4 epsps expression cassette integrated at a single locus within the soybean genome. DNA sequencing analyses of the MON 89788 insert confirm the expected coding region of the cp4 epsps gene cassette, is identical to that transformed in the T-DNA cassette. No backbone sequences from the transformation plasmid were detected and no partial genetic elements, linked or unlinked to the inserted expression cassette were detected.
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LMO characteristics
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  • Food
  • Feed
Additional Information
The soybean line MON98788 was developed to allow for the use of glyphosate, the active ingredient in the herbicide Roundup®, as a weed control option for soybean. This genetically engineered soybean variety contains a glyphosate tolerant form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) isolated from the common soil bacterium, Agrobacterium tumefaciens strain CP4 (CP4 EPSPS).

The EPSPS enzyme is part of the shikimate pathway that is involved in the production of aromatic amino acids and other aromatic compounds in plants. When conventional plants are treated with glyphosate, the plants cannot produce the aromatic amino acids needed to survive. This enzyme is present in all plants, bacteria, fungi, but not in animals, which do not synthesize their own aromatic amino acids. Because the aromatic amino acid biosynthetic pathway is not present in mammalian, avian or aquatic life forms, glyphosate has little if any toxicity for these organisms. The EPSPS enzyme is normally present in food derived from plant and microbial sources.

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