The risk assessment presented was carried out according to the context of the proposed use - that the SPT technology be used in the company's internal operations for seed production. The applicant presents and discusses in detail in her risk assessment some scenarios that could reduce the efficiency of the SPT process in terms of ensuring the absence of the transgene in the offspring. (Proposal: Appendix 7 - Potential scenarios for breaking the genetic insertion 32138 SPT and consequences for the SPT process). These scenarios consider the possibility that the genetic components of the SPT system are separated through genetic recombination mechanisms. According to the discussion presented by the applicant, based on the crossing-over rates, genome location and hemizygous status of the insert, this breakdown of the binding between the gene cassettes of the SPT 32138 insert would be rare events and, if they occur, could be detected and the seeds without compromising the efficiency of the SPT process. The scenarios presented by suppressing one or two of the three genes from the SPT 32138 insert from a single break would originate seeds of different color, which would be removed during the sorting, or would originate plants with phenotypes opposite to those expected (ie male-fertile or male-sterile) plants that would be recognized as off-type plants and eliminated during routine inspection as part of quality control practices. In the extremely rare occurrence of two breaks between the cassettes the elimination of zm-aa1, the proportion of seed observed in each spike in the classification process could indicate an abnormal genotype and the plant would be removed and no longer used. In the case of suppression of the Ms45 and DsRed2 (Alt1) genes, the zm-aa1 gene of the SPT insert would probably pass to the next generation, however, the progeny would be sterile and no longer propagated.