The expression of the novel proteins were studied to assess the likelihood of exposure. The assessors noted that the PAT protein was detected in all tissues, with leaves and flower buds registering a higher amount. On the other hand, the Barnase enzyme was not detected in the root, young and mature leaf, and flower bud tissues. The expression patterns of these novel proteins can be attributed to the promoters controlling the localization of expression. The PssuAt promoter, which drives the expression of the PAT protein, is responsible for the expression in green tissues like leaves. Meanwhile, the pta29 promoter restricts the expression of the Barnase enzyme to the tapetal cell layer during anther development. It was additionally noted that Canola MS8 is male sterile and could not produce any seed, thereby, eliminating the possibility of any sole product of Canola MS8 to be present in food. It was further noted that canola event MS8 is intended to be used as a component in a hybridization system, thus, the regulated article and its products will not enter the food chain. Taken together, exposure to the novel proteins PAT and Barnase is unlikely since these are expressed in tissues that are not used to produce the commerciable product.